Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

Antonio Feliciello; Luca Cardone; Corrado Garbi; Michael D. Ginsberg; Stelio Varrone; Charles S. Rubin; Enrico V. Avvedimento; Max E. Gottesman

doi:10.1016/S0014-5793(99)01585-9

Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

Antonio Feliciello, Luca Cardone, Corrado Garbi, Michael D. Ginsberg, Stelio Varrone, Charles S. Rubin, Enrico V. Avvedimento, Max E. Gottesman

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

Original language	English
Pages (from-to)	174-178
Number of pages	5
Journal	FEBS Letters
Volume	464
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)01585-9
Publication status	Published - Dec 31 1999

Keywords

NMDA receptor
Protein kinase A
Yotiao protein

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0014-5793(99)01585-9

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title = "Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II",

abstract = "A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.",

keywords = "NMDA receptor, Protein kinase A, Yotiao protein",

author = "Antonio Feliciello and Luca Cardone and Corrado Garbi and Ginsberg, {Michael D.} and Stelio Varrone and Rubin, {Charles S.} and Avvedimento, {Enrico V.} and Gottesman, {Max E.}",

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doi = "10.1016/S0014-5793(99)01585-9",

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T1 - Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

AU - Feliciello, Antonio

AU - Cardone, Luca

AU - Garbi, Corrado

AU - Ginsberg, Michael D.

AU - Varrone, Stelio

AU - Rubin, Charles S.

AU - Avvedimento, Enrico V.

AU - Gottesman, Max E.

PY - 1999/12/31

Y1 - 1999/12/31

N2 - A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

AB - A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

KW - NMDA receptor

KW - Protein kinase A

KW - Yotiao protein

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DO - 10.1016/S0014-5793(99)01585-9

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AN - SCOPUS:0033396708

SN - 0014-5793

VL - 464

SP - 174

EP - 178

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

Abstract

Keywords

ASJC Scopus subject areas

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