TY - JOUR
T1 - X-ray crystal structure of the ferric sperm whale myoglobin
T2 - Imidazole complex at 2.0 Å resolution
AU - Lionetti, Claudia
AU - Guanziroli, Maria Grazia
AU - Frigerio, Francesco
AU - Ascenzi, Paolo
AU - Bolognesi, Martino
PY - 1991/2/5
Y1 - 1991/2/5
N2 - The X-ray crystal structure of the ferric sperm whale (Physeter catodon) myoglobin: imidazole complex has been refined at 2.0 Å resolution, to a final R-factor of 14.8%. The overall conformation of the protein is little affected by binding of the ligand. Imidazole is co-ordinated to the heme iron at the distal site, and forces distinguishable local changes in the surrounding protein residues. His64(E7) swings out of the distal pocket and becomes substantially exposed to the solvent; nevertheless, it stabilizes the exogenous ligand by hydrogen bonding. The side-chains of residues Arg45(CD3) and Asp60(E3) are also affected by imidazole association.
AB - The X-ray crystal structure of the ferric sperm whale (Physeter catodon) myoglobin: imidazole complex has been refined at 2.0 Å resolution, to a final R-factor of 14.8%. The overall conformation of the protein is little affected by binding of the ligand. Imidazole is co-ordinated to the heme iron at the distal site, and forces distinguishable local changes in the surrounding protein residues. His64(E7) swings out of the distal pocket and becomes substantially exposed to the solvent; nevertheless, it stabilizes the exogenous ligand by hydrogen bonding. The side-chains of residues Arg45(CD3) and Asp60(E3) are also affected by imidazole association.
UR - http://www.scopus.com/inward/record.url?scp=0026022973&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026022973&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(91)90744-Q
DO - 10.1016/0022-2836(91)90744-Q
M3 - Article
C2 - 1994031
AN - SCOPUS:0026022973
SN - 0022-2836
VL - 217
SP - 409
EP - 412
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 3
ER -