VH3 and VH6 immunoglobulin M repertoire reconstitution after hematopoietic stem-cell transplantation in children

Daniela Di Martino, Paola P. Terranova, Francesca Scuderi, Paola Di Michele, Sofia Iacovone, Lucia Scarso, Sandro Dallorso, Giorgio Dini, Giuseppe Morreale, Angelo Valetto

Research output: Contribution to journalArticlepeer-review


Background. Immune reconstitution after hematopoietic stem-cell transplantation (HSCT) occurs gradually. Thus, a variable period of immunodeficiency may be present, leading to immunomediated complications, such as graft-versus-host disease (GVHD) and opportunistic infections. Methods. To better understand the kinetics of B-cell repertoire reconstitution in children, 49 pediatric patients were analyzed before and after transplantation by immunoglobulin (Ig) HCDR3 fingerprinting, which is a molecular technique that analyzes one of the hypervariable segments of the Ig heavy chain, which provides the amino acid residues that are essential to interact with antigens. Results. In healthy donors, the CDR3 fingerprinting profile shows 16 to 20 bands, and each band corresponds to a particular length of CDR3. This situation is considered polyclonal. Patients analyzed just after transplantation show strong oligoclonality, because only a few CDR3 bands are detected within the first 3 to 6 months. Conclusions. The authors' data show a significant lag in diversification of the B-cell repertoire, which reaches the polyclonal situation of normal healthy donors approximately 6 months after HSCT. This period may vary depending on the type of transplant (autologous vs. allogeneic) and on the immunosuppressive therapy related to GVHD.

Original languageEnglish
Pages (from-to)98-107
Number of pages10
Issue number1
Publication statusPublished - Jan 15 2005


  • Allogeneic and autologous transplantation
  • CDR3
  • Immunologic reconstitution
  • Silver staining

ASJC Scopus subject areas

  • Transplantation
  • Immunology


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