TY - JOUR
T1 - Upregulation of ICAM-1 expression in the conjunctiva of patients with chronic graft-versus-host disease
AU - Aronni, S.
AU - Cortes, M.
AU - Sacchetti, M.
AU - Lambiase, A.
AU - Micera, A.
AU - Sgrulletta, R.
AU - Bonini, Stefano
PY - 2006/1
Y1 - 2006/1
N2 - PURPOSE. To correlate conjunctival intercellular adhesion molecule 1 (ICAM-1) expression with cytologic and clinical findings of chronic graft-versus-host disease (GVHD). METHODS. Seven patients with chronic GVHD-related keratoconjunctivitis and five age-matched normal controls were recruited for the study. Clinical examination included medical history, visual acuity, evaluation of ocular signs and symptoms (scored from 0 to 3), corneal fluorescein staining (scored from 0 to 5 on the basis of the number of corneal sectors involved), Schirmer test type I, and break-up time (BUT). Impression cytology samples were collected from the nasal and inferior bulbar conjunctiva of patients and controls. Goblet cells were counted in three randomly selected fields and averaged. Immunofluorescent staining for ICAM-1 was carried out and the percentage of cells expressing the marker was evaluated. RESULTS. All patients showed signs and symptoms of keratoconjunctivitis sicca. Schirmer test type I and BUT were reduced (4.8±6.7 mm/5 min and 3.9±2.7 seconds, respectively). Goblet cells were significantly reduced in GVHD eyes with respect to normal eyes (65±30.5 and 192±16.9 cells/field respectively; p
AB - PURPOSE. To correlate conjunctival intercellular adhesion molecule 1 (ICAM-1) expression with cytologic and clinical findings of chronic graft-versus-host disease (GVHD). METHODS. Seven patients with chronic GVHD-related keratoconjunctivitis and five age-matched normal controls were recruited for the study. Clinical examination included medical history, visual acuity, evaluation of ocular signs and symptoms (scored from 0 to 3), corneal fluorescein staining (scored from 0 to 5 on the basis of the number of corneal sectors involved), Schirmer test type I, and break-up time (BUT). Impression cytology samples were collected from the nasal and inferior bulbar conjunctiva of patients and controls. Goblet cells were counted in three randomly selected fields and averaged. Immunofluorescent staining for ICAM-1 was carried out and the percentage of cells expressing the marker was evaluated. RESULTS. All patients showed signs and symptoms of keratoconjunctivitis sicca. Schirmer test type I and BUT were reduced (4.8±6.7 mm/5 min and 3.9±2.7 seconds, respectively). Goblet cells were significantly reduced in GVHD eyes with respect to normal eyes (65±30.5 and 192±16.9 cells/field respectively; p
KW - Conjunctiva
KW - Graft-versus-host disease
KW - ICAM-1
KW - Impression cytology
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M3 - Article
C2 - 16496240
AN - SCOPUS:33645538302
SN - 1120-6721
VL - 16
SP - 17
EP - 23
JO - European Journal of Ophthalmology
JF - European Journal of Ophthalmology
IS - 1
ER -