TY - JOUR
T1 - Up-regulation of the monocyte chemotactic protein-3 in sera from bone marrow transplanted children with torquetenovirus infection
AU - Zanotta, Nunzia
AU - Maximova, Natalia
AU - Campisciano, Giuseppina
AU - Del Savio, Rossella
AU - Pizzol, Antonio
AU - Casalicchio, Giorgia
AU - Berton, Emanuela
AU - Comar, Manola
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Background: Torquetenovirus (TTV) represents a commensal human virus producing life-long viremia in approximately 80% of healthy individuals of all ages. A potential pathogenic role for TTV has been suggested in immunocompromised patients with hepatitis of unknown etiology sustained by strong proinflammatory cytokines. Objectives: The aim of this study was to investigate the sera immunological profile linked to TTV infection in bone marrow transplant (BMT) children with liver injury. Study design: TTV infection was assessed in sera from 27 BMT patients with altered hepatic parameters and histological features, by the use of quantitative real-time PCR, along with TTV genogroups and coinfection with HEV. The qualitative and quantitative nature of soluble inflammatory factors was evaluated studying a large set of cytokines using the Bioplex platform. As controls, sera from 22 healthy children negative for serological and molecular hepatitis markers including TTV and HEV, and for autoimmune diseases, were selected. Results and conclusions: TTV was detected in 81.4% of BMT patients with a viral load ranging from 105 to 109 copies/mL. All samples were HEV-RNA negative. A pattern of cytokines, IFN-γ, TNF-α, FGF-basic (p
AB - Background: Torquetenovirus (TTV) represents a commensal human virus producing life-long viremia in approximately 80% of healthy individuals of all ages. A potential pathogenic role for TTV has been suggested in immunocompromised patients with hepatitis of unknown etiology sustained by strong proinflammatory cytokines. Objectives: The aim of this study was to investigate the sera immunological profile linked to TTV infection in bone marrow transplant (BMT) children with liver injury. Study design: TTV infection was assessed in sera from 27 BMT patients with altered hepatic parameters and histological features, by the use of quantitative real-time PCR, along with TTV genogroups and coinfection with HEV. The qualitative and quantitative nature of soluble inflammatory factors was evaluated studying a large set of cytokines using the Bioplex platform. As controls, sera from 22 healthy children negative for serological and molecular hepatitis markers including TTV and HEV, and for autoimmune diseases, were selected. Results and conclusions: TTV was detected in 81.4% of BMT patients with a viral load ranging from 105 to 109 copies/mL. All samples were HEV-RNA negative. A pattern of cytokines, IFN-γ, TNF-α, FGF-basic (p
KW - Bone marrow transplanted children
KW - Liver injury
KW - Monocyte chemotactic protein-3
KW - Torquetenovirus infection
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U2 - 10.1016/j.jcv.2014.11.028
DO - 10.1016/j.jcv.2014.11.028
M3 - Article
C2 - 25600596
AN - SCOPUS:84921283474
SN - 1386-6532
VL - 63
SP - 6
EP - 11
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
ER -