The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.
|Number of pages||3|
|Journal||Clinical and Vaccine Immunology|
|Publication status||Published - Mar 2006|
ASJC Scopus subject areas
- Clinical Biochemistry
- Immunology and Allergy
- Microbiology (medical)