Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

S. E. Burastero; C. Paolucci; D. Breda; G. Monasterolo; R. E. Rossi; L. Vangelista

doi:10.1128/CVI.13.3.420-422.2006

Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

S. E. Burastero, C. Paolucci, D. Breda, G. Monasterolo, R. E. Rossi, L. Vangelista

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Original language	English
Pages (from-to)	420-422
Number of pages	3
Journal	Clinical and Vaccine Immunology
Volume	13
Issue number	3
DOIs	https://doi.org/10.1128/CVI.13.3.420-422.2006
Publication status	Published - Mar 2006

ASJC Scopus subject areas

Clinical Biochemistry
Immunology
Immunology and Allergy
Microbiology (medical)

Access to Document

10.1128/CVI.13.3.420-422.2006

Cite this

@article{a72b710e8b3b414fb5b4cb58d000f71d,

title = "Unreliable measurement of basophil maximum leukotriene release with the B{\"u}hlmann CAST 2000 enzyme-linked immunosorbent assay kit",

abstract = "The B{\"u}hlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.",

author = "Burastero, {S. E.} and C. Paolucci and D. Breda and G. Monasterolo and Rossi, {R. E.} and L. Vangelista",

year = "2006",

month = mar,

doi = "10.1128/CVI.13.3.420-422.2006",

language = "English",

volume = "13",

pages = "420--422",

journal = "Clinical and Vaccine Immunology",

issn = "1556-6811",

publisher = "American Society for Microbiology",

number = "3",

}

TY - JOUR

T1 - Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

AU - Burastero, S. E.

AU - Paolucci, C.

AU - Breda, D.

AU - Monasterolo, G.

AU - Rossi, R. E.

AU - Vangelista, L.

PY - 2006/3

Y1 - 2006/3

N2 - The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

AB - The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

UR - http://www.scopus.com/inward/record.url?scp=33645538809&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645538809&partnerID=8YFLogxK

U2 - 10.1128/CVI.13.3.420-422.2006

DO - 10.1128/CVI.13.3.420-422.2006

M3 - Article

C2 - 16522787

AN - SCOPUS:33645538809

SN - 1556-6811

VL - 13

SP - 420

EP - 422

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 3

ER -

Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this