Transplantation of undifferentiated human mesenchymal stem cells protects against 6-hydroxydopamine neurotoxicity in the rat

Fabio Blandini, Lidia Cova, Marie Therese Armentero, Eleonora Zennaro, Giovanna Levandis, Patrizia Bossolasco, Cinzia Calzarossa, Manuela Mellone, Busca Giuseppe, Giorgio Lambertenghi Deliliers, Elio Polli, Giuseppe Nappi, Vincenzo Silani

Research output: Contribution to journalArticlepeer-review


Stem cells have been increasingly recognized as a potential tool to replace or support cells damaged by the neurodegenerative process that underlies Parkinson's disease (PD). In this frame, human adult mesenchymal stem cells (hMSCs) have been proposed as an attractive alternative to heterologous embryonic or neural precursor cells. To address this issue, in this study we implanted undifferentiated hMSCs into the striatum of rats bearing a lesion of the nigrostriatal pathway induced by local injection of 6-hydroxydopamine (6-OHDA), a widely recognized rodent model of PD. Before grafting, cultured hMSCs expressed markers of both undifferentiated and committed neural cells, including nestin, GAP-43, NSE, β-tubulin III, and MAP-2, as well as several cytokine mRNAs. No glial or specific neuronal markers were detected. Following transplantation, some hMSCs acquired a glial-like phenotype, as shown by immunoreactivity for glial fibrillary acid protein (GFAP), but only in animals bearing the nigrostriatal lesion. More importantly, rats that received the striatal graft showed increased survival of both cell bodies and terminals of dopaminergic, nigrostriatal neurons, coupled with a reduction of the behavioral abnormalities (apomorphine-induced turning behavior) associated with the lesion. No differentiation of the MSCs toward a neuronal (dopaminergic) phenotype was observed in vivo. In conclusion, our results suggest that grafted hMSCs exert neuroprotective effects against nigrostriatal degeneration induced by 6-OHDA. The mechanisms underlying this effect remain to be clarified, although it is likely that the acquisition of a glial phenotype by grafted hMSCs may lead to the release of prosurvival cytokines within the lesioned striatum.

Original languageEnglish
Pages (from-to)203-217
Number of pages15
JournalCell Transplantation
Issue number2
Publication statusPublished - 2010


  • 6-hydroxydopamine
  • Cytokines
  • Graft
  • Neuroprotection
  • Rat
  • Striatum
  • Substantia nigra

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering


Dive into the research topics of 'Transplantation of undifferentiated human mesenchymal stem cells protects against 6-hydroxydopamine neurotoxicity in the rat'. Together they form a unique fingerprint.

Cite this