Transient proteasome inhibition as a strategy to enhance lentiviral transduction of hematopoietic CD34 + cells and T lymphocytes: Implications for the use of low viral doses and large-size vectors

Valeria Leuci; Giulia Mesiano; Loretta Gammaitoni; Cristina Cammarata; Sonia Capellero; Maja Todorovic; Noela Jordaney; Paola Circosta; Angela Elia; Marina Lesnikova; George E. Georges; Wanda Piacibello; Franca Fagioli; Alessandro Cignetti; Massimo Aglietta; Dario Sangiolo

doi:10.1016/j.jbiotec.2011.09.001

Transient proteasome inhibition as a strategy to enhance lentiviral transduction of hematopoietic CD34 + cells and T lymphocytes: Implications for the use of low viral doses and large-size vectors

Valeria Leuci, Giulia Mesiano, Loretta Gammaitoni, Cristina Cammarata, Sonia Capellero, Maja Todorovic, Noela Jordaney, Paola Circosta, Angela Elia, Marina Lesnikova, George E. Georges, Wanda Piacibello, Franca Fagioli, Alessandro Cignetti, Massimo Aglietta, Dario Sangiolo

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article › peer-review

Abstract

The proteasome system restricts lentiviral transduction of stem cells. We exploited proteasome inhibition as a strategy to enhance transduction of both hematopoietic stem cells (HSC) and T lymphocytes with low dose or large-size lentiviral vectors (LV). HSC showed higher transduction efficiency if transiently exposed to proteasome inhibitor MG132 (41.8% vs 10.7%, p

Original language	English
Pages (from-to)	218-226
Number of pages	9
Journal	Journal of Biotechnology
Volume	156
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiotec.2011.09.001
Publication status	Published - Dec 10 2011

Keywords

Hematopoietic stem cells
Lentiviral transduction
T lymphocytes
Transduction efficiency

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/j.jbiotec.2011.09.001

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Leuci, V., Mesiano, G., Gammaitoni, L., Cammarata, C., Capellero, S., Todorovic, M., Jordaney, N., Circosta, P., Elia, A., Lesnikova, M., Georges, G. E., Piacibello, W., Fagioli, F., Cignetti, A., Aglietta, M., & Sangiolo, D. (2011). Transient proteasome inhibition as a strategy to enhance lentiviral transduction of hematopoietic CD34 + cells and T lymphocytes: Implications for the use of low viral doses and large-size vectors. Journal of Biotechnology, 156(3), 218-226. https://doi.org/10.1016/j.jbiotec.2011.09.001

Transient proteasome inhibition as a strategy to enhance lentiviral transduction of hematopoietic CD34 + cells and T lymphocytes : Implications for the use of low viral doses and large-size vectors. / Leuci, Valeria; Mesiano, Giulia; Gammaitoni, Loretta et al.

In: Journal of Biotechnology, Vol. 156, No. 3, 10.12.2011, p. 218-226.

Research output: Contribution to journal › Article › peer-review

Leuci, V, Mesiano, G, Gammaitoni, L, Cammarata, C, Capellero, S, Todorovic, M, Jordaney, N, Circosta, P, Elia, A, Lesnikova, M, Georges, GE, Piacibello, W, Fagioli, F, Cignetti, A, Aglietta, M & Sangiolo, D 2011, 'Transient proteasome inhibition as a strategy to enhance lentiviral transduction of hematopoietic CD34 + cells and T lymphocytes: Implications for the use of low viral doses and large-size vectors', Journal of Biotechnology, vol. 156, no. 3, pp. 218-226. https://doi.org/10.1016/j.jbiotec.2011.09.001

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abstract = "The proteasome system restricts lentiviral transduction of stem cells. We exploited proteasome inhibition as a strategy to enhance transduction of both hematopoietic stem cells (HSC) and T lymphocytes with low dose or large-size lentiviral vectors (LV). HSC showed higher transduction efficiency if transiently exposed to proteasome inhibitor MG132 (41.8% vs 10.7%, p",

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AU - Capellero, Sonia

AU - Todorovic, Maja

AU - Jordaney, Noela

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AU - Elia, Angela

AU - Lesnikova, Marina

AU - Georges, George E.

AU - Piacibello, Wanda

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AU - Cignetti, Alessandro

AU - Aglietta, Massimo

AU - Sangiolo, Dario

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Transient proteasome inhibition as a strategy to enhance lentiviral transduction of hematopoietic CD34 + cells and T lymphocytes: Implications for the use of low viral doses and large-size vectors

Abstract

Keywords

ASJC Scopus subject areas

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