TY - JOUR
T1 - Transcribed-ultra conserved region expression is associated with outcome in high-risk neuroblastoma
AU - Scaruffi, Paola
AU - Stigliani, Sara
AU - Moretti, Stefano
AU - Coco, Simona
AU - De Vecchi, Carla
AU - Valdora, Francesca
AU - Garaventa, Alberto
AU - Bonassi, Stefano
AU - Tonini, Gian P.
PY - 2009/12/15
Y1 - 2009/12/15
N2 - Background: Neuroblastoma is the most common, pediatric, extra-cranial, malignant solid tumor. Despite multimodal therapeutic protocols, outcome for children with a high-risk clinical phenotype remains poor, with long-term survival still less than 40%. Hereby, we evaluated the potential of non-coding RNA expression to predict outcome in high-risk, stage 4 neuroblastoma.Methods: We analyzed expression of 481 Ultra Conserved Regions (UCRs) by reverse transcription-quantitative real-time PCR and of 723 microRNAs by microarrays in 34 high-risk, stage 4 neuroblastoma patients.Results: First, the comparison of 8 short- versus 12 long-term survivors showed that 54 UCRs were significantly (P <0.0491) over-expressed in the former group. For 48 Ultra Conserved Region (UCRs) the expression levels above the cut-off values defined by ROC curves were strongly associated with good-outcome (OS: 0.0001
AB - Background: Neuroblastoma is the most common, pediatric, extra-cranial, malignant solid tumor. Despite multimodal therapeutic protocols, outcome for children with a high-risk clinical phenotype remains poor, with long-term survival still less than 40%. Hereby, we evaluated the potential of non-coding RNA expression to predict outcome in high-risk, stage 4 neuroblastoma.Methods: We analyzed expression of 481 Ultra Conserved Regions (UCRs) by reverse transcription-quantitative real-time PCR and of 723 microRNAs by microarrays in 34 high-risk, stage 4 neuroblastoma patients.Results: First, the comparison of 8 short- versus 12 long-term survivors showed that 54 UCRs were significantly (P <0.0491) over-expressed in the former group. For 48 Ultra Conserved Region (UCRs) the expression levels above the cut-off values defined by ROC curves were strongly associated with good-outcome (OS: 0.0001
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U2 - 10.1186/1471-2407-9-441
DO - 10.1186/1471-2407-9-441
M3 - Article
C2 - 20003513
AN - SCOPUS:74249097855
SN - 1471-2407
VL - 9
JO - BMC Cancer
JF - BMC Cancer
M1 - 441
ER -