Towards a 3D culture of mouse ovarian follicles

Martina Belli; Giulia Vigone; Valeria Merico; Carlo Alberto Redi; Maurizio Zuccotti; Silvia Garagna

doi:10.1387/ijdb.120175mz

Towards a 3D culture of mouse ovarian follicles

Martina Belli, Giulia Vigone, Valeria Merico, Carlo Alberto Redi, Maurizio Zuccotti, Silvia Garagna

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tightly connected follicle cells that mediate the action of external signals and nourish the gamete during its maturation. Thus, the maintenance of follicle organization during the whole growth process is crucial for the correct acquisition of developmental competence. In recent years, much attention has been given to in vitro culture systems capable of maintaining follicle architecture. With the aim of providing a quick reference guide, in this review we will summarize the techniques developed for the 3D culture of mouse follicles.

Original language	English
Pages (from-to)	931-937
Number of pages	7
Journal	International Journal of Developmental Biology
Volume	56
Issue number	10-12
DOIs	https://doi.org/10.1387/ijdb.120175mz
Publication status	Published - 2012

Keywords

3D culture
Follicle architecture
Follicle organization
Matrix
Ovarian follicle

ASJC Scopus subject areas

Developmental Biology
Embryology

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10.1387/ijdb.120175mz

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AU - Merico, Valeria

AU - Redi, Carlo Alberto

AU - Zuccotti, Maurizio

AU - Garagna, Silvia

PY - 2012

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AB - The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tightly connected follicle cells that mediate the action of external signals and nourish the gamete during its maturation. Thus, the maintenance of follicle organization during the whole growth process is crucial for the correct acquisition of developmental competence. In recent years, much attention has been given to in vitro culture systems capable of maintaining follicle architecture. With the aim of providing a quick reference guide, in this review we will summarize the techniques developed for the 3D culture of mouse follicles.

KW - 3D culture

KW - Follicle architecture

KW - Follicle organization

KW - Matrix

KW - Ovarian follicle

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Towards a 3D culture of mouse ovarian follicles

Abstract

Keywords

ASJC Scopus subject areas

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