TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons

Francesca Grassi; Anna Maria Mileo; Lucia Monaco; Antonello Punturieri; Angela Santoni; Fabrizio Eusebi

doi:10.1016/0006-8993(94)90883-4

TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons

Francesca Grassi, Anna Maria Mileo, Lucia Monaco, Antonello Punturieri, Angela Santoni, Fabrizio Eusebi

Research output: Contribution to journal › Article › peer-review

Abstract

Tumor necrosis factor-α (TNF-α) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-α receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-α effect are discussed.

Original language	English
Pages (from-to)	226-230
Number of pages	5
Journal	Brain Research
Volume	659
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(94)90883-4
Publication status	Published - Oct 3 1994

Keywords

Cultured hippocampal neuron
Miniature synaptic current
Spontaneous synaptic activity
TNF receptor
Tumor necrosis factor-α

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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title = "TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons",

abstract = "Tumor necrosis factor-α (TNF-α) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-α receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-α effect are discussed.",

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AU - Grassi, Francesca

AU - Mileo, Anna Maria

AU - Monaco, Lucia

AU - Punturieri, Antonello

AU - Santoni, Angela

AU - Eusebi, Fabrizio

PY - 1994/10/3

Y1 - 1994/10/3

N2 - Tumor necrosis factor-α (TNF-α) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-α receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-α effect are discussed.

AB - Tumor necrosis factor-α (TNF-α) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-α receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-α effect are discussed.

KW - Cultured hippocampal neuron

KW - Miniature synaptic current

KW - Spontaneous synaptic activity

KW - TNF receptor

KW - Tumor necrosis factor-α

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TNF-α increases the frequency of spontaneous miniature synaptic currents in cultured rat hippocampal neurons

Abstract

Keywords

ASJC Scopus subject areas

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