Therapeutic efficacy of intraperitoneal polymyxin B and polymyxin-like peptides alone or combined with levofloxacin in rat models of septic shock

Andrea Giacometti; Oscar Cirioni; Roberto Ghiselli; Federico Mocchegiani; Alessandra Mataloni Paggi; Fiorenza Orlando; Wojciech Kamysz; Franciszek Kasprzykowski; Zbigniew Maćkiewicz; Giorgio Scalise; Vittorio Saba

Therapeutic efficacy of intraperitoneal polymyxin B and polymyxin-like peptides alone or combined with levofloxacin in rat models of septic shock

Andrea Giacometti, Oscar Cirioni, Roberto Ghiselli, Federico Mocchegiani, Alessandra Mataloni Paggi, Fiorenza Orlando, Wojciech Kamysz, Franciszek Kasprzykowski, Zbigniew Maćkiewicz, Giorgio Scalise, Vittorio Saba

Istituto Nazionale di Riposo e Cura per Anziani V.E. II

Research output: Contribution to journal › Article › peer-review

Abstract

The efficacy of two polymyxin-like peptides, KFFKFFKFF and IKFLKFLKFL, alone and combined with levofloxacin, was investigated in a rat model of septic shock. Rats were given an ip injection of 2 × 10¹⁰ cfu of Escherichia coli and randomized to receive ip isotonic sodium chloride solution, 7 mg/kg levofloxacin, 1 mg/kg polymyxin B and 1 mg/kg of each polymyxin-like peptides alone or combined with 7 mg/kg levofloxacin. Polymyxins achieved a significant reduction in plasma endotoxin and tumour necrosis factor α (TNF-α) concentration. Levofloxacin significantly reduced the bacterial growth and TNF-α concentration. The combinations of polymyxin-like peptides and levofloxacin demonstrated the highest efficacy.

Original language	English
Pages (from-to)	193-196
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	49
Issue number	1
Publication status	Published - 2002

ASJC Scopus subject areas

Microbiology
Pharmacology

Cite this

@article{efcdf024588a467b803e8de79755ce18,

title = "Therapeutic efficacy of intraperitoneal polymyxin B and polymyxin-like peptides alone or combined with levofloxacin in rat models of septic shock",

abstract = "The efficacy of two polymyxin-like peptides, KFFKFFKFF and IKFLKFLKFL, alone and combined with levofloxacin, was investigated in a rat model of septic shock. Rats were given an ip injection of 2 × 1010 cfu of Escherichia coli and randomized to receive ip isotonic sodium chloride solution, 7 mg/kg levofloxacin, 1 mg/kg polymyxin B and 1 mg/kg of each polymyxin-like peptides alone or combined with 7 mg/kg levofloxacin. Polymyxins achieved a significant reduction in plasma endotoxin and tumour necrosis factor α (TNF-α) concentration. Levofloxacin significantly reduced the bacterial growth and TNF-α concentration. The combinations of polymyxin-like peptides and levofloxacin demonstrated the highest efficacy.",

author = "Andrea Giacometti and Oscar Cirioni and Roberto Ghiselli and Federico Mocchegiani and Paggi, {Alessandra Mataloni} and Fiorenza Orlando and Wojciech Kamysz and Franciszek Kasprzykowski and Zbigniew Ma{\'c}kiewicz and Giorgio Scalise and Vittorio Saba",

year = "2002",

language = "English",

volume = "49",

pages = "193--196",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Therapeutic efficacy of intraperitoneal polymyxin B and polymyxin-like peptides alone or combined with levofloxacin in rat models of septic shock

AU - Giacometti, Andrea

AU - Cirioni, Oscar

AU - Ghiselli, Roberto

AU - Mocchegiani, Federico

AU - Paggi, Alessandra Mataloni

AU - Orlando, Fiorenza

AU - Kamysz, Wojciech

AU - Kasprzykowski, Franciszek

AU - Maćkiewicz, Zbigniew

AU - Scalise, Giorgio

AU - Saba, Vittorio

PY - 2002

Y1 - 2002

N2 - The efficacy of two polymyxin-like peptides, KFFKFFKFF and IKFLKFLKFL, alone and combined with levofloxacin, was investigated in a rat model of septic shock. Rats were given an ip injection of 2 × 1010 cfu of Escherichia coli and randomized to receive ip isotonic sodium chloride solution, 7 mg/kg levofloxacin, 1 mg/kg polymyxin B and 1 mg/kg of each polymyxin-like peptides alone or combined with 7 mg/kg levofloxacin. Polymyxins achieved a significant reduction in plasma endotoxin and tumour necrosis factor α (TNF-α) concentration. Levofloxacin significantly reduced the bacterial growth and TNF-α concentration. The combinations of polymyxin-like peptides and levofloxacin demonstrated the highest efficacy.

AB - The efficacy of two polymyxin-like peptides, KFFKFFKFF and IKFLKFLKFL, alone and combined with levofloxacin, was investigated in a rat model of septic shock. Rats were given an ip injection of 2 × 1010 cfu of Escherichia coli and randomized to receive ip isotonic sodium chloride solution, 7 mg/kg levofloxacin, 1 mg/kg polymyxin B and 1 mg/kg of each polymyxin-like peptides alone or combined with 7 mg/kg levofloxacin. Polymyxins achieved a significant reduction in plasma endotoxin and tumour necrosis factor α (TNF-α) concentration. Levofloxacin significantly reduced the bacterial growth and TNF-α concentration. The combinations of polymyxin-like peptides and levofloxacin demonstrated the highest efficacy.

UR - http://www.scopus.com/inward/record.url?scp=0036156219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036156219&partnerID=8YFLogxK

M3 - Article

C2 - 11751788

AN - SCOPUS:0036156219

SN - 0305-7453

VL - 49

SP - 193

EP - 196

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 1

ER -

Therapeutic efficacy of intraperitoneal polymyxin B and polymyxin-like peptides alone or combined with levofloxacin in rat models of septic shock

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this