The phosphorylation pattern of bovine heart complex I subunits

Giuseppe Palmisano; Anna Maria Sardanelli; Anna Signorile; Sergio Papa; Martin R. Larsen

doi:10.1002/pmic.200600801

The phosphorylation pattern of bovine heart complex I subunits

Giuseppe Palmisano, Anna Maria Sardanelli, Anna Signorile, Sergio Papa, Martin R. Larsen

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

The phosphoproteome of bovine heart complex I of the respiratory chain has been analysed with a procedure based on nondenaturing gel electrophoretic separation of complex I from small quantities of mitochondria samples, in-gel digestion, in combination with phosphopeptide enrichment by titanium dioxide and MS. The results, complemented by analyses of purified samples of complex I, showed phosphorylation of five subunits of the complex, 42 kDa (human gene NDUFA10), ESSS, B14.5a (human gene NDUFA7), B14.5b (human gene NDUFC2) and B16.6 (GRIM-19). MS also revealed the presence of phosphorylated programmed cell death protein 8(AIF) in native and purified samples of complex I analysed. The possible physiological relevance of these findings is discussed.

Original language	English
Pages (from-to)	1575-1583
Number of pages	9
Journal	Proteomics
Volume	7
Issue number	10
DOIs	https://doi.org/10.1002/pmic.200600801
Publication status	Published - May 2007

Keywords

Complex I
Mitochondria
Phosphoproteomics

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1002/pmic.200600801

Cite this

@article{1b6f7f6f67354e88a09c056d61094c74,

title = "The phosphorylation pattern of bovine heart complex I subunits",

abstract = "The phosphoproteome of bovine heart complex I of the respiratory chain has been analysed with a procedure based on nondenaturing gel electrophoretic separation of complex I from small quantities of mitochondria samples, in-gel digestion, in combination with phosphopeptide enrichment by titanium dioxide and MS. The results, complemented by analyses of purified samples of complex I, showed phosphorylation of five subunits of the complex, 42 kDa (human gene NDUFA10), ESSS, B14.5a (human gene NDUFA7), B14.5b (human gene NDUFC2) and B16.6 (GRIM-19). MS also revealed the presence of phosphorylated programmed cell death protein 8(AIF) in native and purified samples of complex I analysed. The possible physiological relevance of these findings is discussed.",

keywords = "Complex I, Mitochondria, Phosphoproteomics",

author = "Giuseppe Palmisano and Sardanelli, {Anna Maria} and Anna Signorile and Sergio Papa and Larsen, {Martin R.}",

year = "2007",

month = may,

doi = "10.1002/pmic.200600801",

language = "English",

volume = "7",

pages = "1575--1583",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag",

number = "10",

}

TY - JOUR

T1 - The phosphorylation pattern of bovine heart complex I subunits

AU - Palmisano, Giuseppe

AU - Sardanelli, Anna Maria

AU - Signorile, Anna

AU - Papa, Sergio

AU - Larsen, Martin R.

PY - 2007/5

Y1 - 2007/5

N2 - The phosphoproteome of bovine heart complex I of the respiratory chain has been analysed with a procedure based on nondenaturing gel electrophoretic separation of complex I from small quantities of mitochondria samples, in-gel digestion, in combination with phosphopeptide enrichment by titanium dioxide and MS. The results, complemented by analyses of purified samples of complex I, showed phosphorylation of five subunits of the complex, 42 kDa (human gene NDUFA10), ESSS, B14.5a (human gene NDUFA7), B14.5b (human gene NDUFC2) and B16.6 (GRIM-19). MS also revealed the presence of phosphorylated programmed cell death protein 8(AIF) in native and purified samples of complex I analysed. The possible physiological relevance of these findings is discussed.

AB - The phosphoproteome of bovine heart complex I of the respiratory chain has been analysed with a procedure based on nondenaturing gel electrophoretic separation of complex I from small quantities of mitochondria samples, in-gel digestion, in combination with phosphopeptide enrichment by titanium dioxide and MS. The results, complemented by analyses of purified samples of complex I, showed phosphorylation of five subunits of the complex, 42 kDa (human gene NDUFA10), ESSS, B14.5a (human gene NDUFA7), B14.5b (human gene NDUFC2) and B16.6 (GRIM-19). MS also revealed the presence of phosphorylated programmed cell death protein 8(AIF) in native and purified samples of complex I analysed. The possible physiological relevance of these findings is discussed.

KW - Complex I

KW - Mitochondria

KW - Phosphoproteomics

UR - http://www.scopus.com/inward/record.url?scp=34249736347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249736347&partnerID=8YFLogxK

U2 - 10.1002/pmic.200600801

DO - 10.1002/pmic.200600801

M3 - Article

C2 - 17443843

AN - SCOPUS:34249736347

SN - 1615-9853

VL - 7

SP - 1575

EP - 1583

JO - Proteomics

JF - Proteomics

IS - 10

ER -

The phosphorylation pattern of bovine heart complex I subunits

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this