The phosphorylation pattern of bovine heart complex I subunits

Giuseppe Palmisano, Anna Maria Sardanelli, Anna Signorile, Sergio Papa, Martin R. Larsen

Research output: Contribution to journalArticlepeer-review


The phosphoproteome of bovine heart complex I of the respiratory chain has been analysed with a procedure based on nondenaturing gel electrophoretic separation of complex I from small quantities of mitochondria samples, in-gel digestion, in combination with phosphopeptide enrichment by titanium dioxide and MS. The results, complemented by analyses of purified samples of complex I, showed phosphorylation of five subunits of the complex, 42 kDa (human gene NDUFA10), ESSS, B14.5a (human gene NDUFA7), B14.5b (human gene NDUFC2) and B16.6 (GRIM-19). MS also revealed the presence of phosphorylated programmed cell death protein 8(AIF) in native and purified samples of complex I analysed. The possible physiological relevance of these findings is discussed.

Original languageEnglish
Pages (from-to)1575-1583
Number of pages9
Issue number10
Publication statusPublished - May 2007


  • Complex I
  • Mitochondria
  • Phosphoproteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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