The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex

L. Verduci; M. Ferraiuolo; A. Sacconi; F. Ganci; J. Vitale; T. Colombo; P. Paci; S. Strano; G. Macino; N. Rajewsky; G. Blandino

doi:10.1186/s13059-017-1368-y [doi]

The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex

L. Verduci, M. Ferraiuolo, A. Sacconi, F. Ganci, J. Vitale, T. Colombo, P. Paci, S. Strano, G. Macino, N. Rajewsky, G. Blandino

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Circular RNAs are a class of endogenous RNAs with various functions in eukaryotic cells. Worthy of note, circular RNAs play a critical role in cancer. Currently, nothing is known about their role in head and neck squamous cell carcinoma (HNSCC). The identification of circular RNAs in HNSCC might become useful for diagnostic and therapeutic strategies in HNSCC. RESULTS: Using samples from 115 HNSCC patients, we find that circPVT1 is over-expressed in tumors compared to matched non-tumoral tissues, with particular enrichment in patients with TP53 mutations. circPVT1 up- and down-regulation determine, respectively, an increase and a reduction of the malignant phenotype in HNSCC cell lines. We show that circPVT1 expression is transcriptionally enhanced by the mut-p53/YAP/TEAD complex. circPVT1 acts as an oncogene modulating the expression of miR-497-5p and genes involved in the control of cell proliferation. CONCLUSIONS: This study shows the oncogenic role of circPVT1 in HNSCC, extending current knowledge about the role of circular RNAs in cancer.

Original language	English
Article number	237
Pages (from-to)	1-24
Number of pages	24
Journal	Genome Biology
Volume	18
Issue number	1
DOIs	https://doi.org/10.1186/s13059-017-1368-y [doi]
Publication status	Published - Dec 20 2017

Keywords

HNSCC
PVT1
TEAD
YAP
circPVT1
miR-497-5p
mut-p53

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10.1186/s13059-017-1368-y [doi]

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Verduci, L., Ferraiuolo, M., Sacconi, A., Ganci, F., Vitale, J., Colombo, T., Paci, P., Strano, S., Macino, G., Rajewsky, N., & Blandino, G. (2017). The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex. Genome Biology, 18(1), 1-24. [237]. https://doi.org/10.1186/s13059-017-1368-y [doi]

Verduci, L, Ferraiuolo, M, Sacconi, A , Ganci, F, Vitale, J, Colombo, T, Paci, P, Strano, S, Macino, G, Rajewsky, N & Blandino, G 2017, 'The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex', Genome Biology, vol. 18, no. 1, 237, pp. 1-24. https://doi.org/10.1186/s13059-017-1368-y [doi]

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title = "The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex",

abstract = "BACKGROUND: Circular RNAs are a class of endogenous RNAs with various functions in eukaryotic cells. Worthy of note, circular RNAs play a critical role in cancer. Currently, nothing is known about their role in head and neck squamous cell carcinoma (HNSCC). The identification of circular RNAs in HNSCC might become useful for diagnostic and therapeutic strategies in HNSCC. RESULTS: Using samples from 115 HNSCC patients, we find that circPVT1 is over-expressed in tumors compared to matched non-tumoral tissues, with particular enrichment in patients with TP53 mutations. circPVT1 up- and down-regulation determine, respectively, an increase and a reduction of the malignant phenotype in HNSCC cell lines. We show that circPVT1 expression is transcriptionally enhanced by the mut-p53/YAP/TEAD complex. circPVT1 acts as an oncogene modulating the expression of miR-497-5p and genes involved in the control of cell proliferation. CONCLUSIONS: This study shows the oncogenic role of circPVT1 in HNSCC, extending current knowledge about the role of circular RNAs in cancer.",

keywords = "HNSCC, PVT1, TEAD, YAP, circPVT1, miR-497-5p, mut-p53",

author = "L. Verduci and M. Ferraiuolo and A. Sacconi and F. Ganci and J. Vitale and T. Colombo and P. Paci and S. Strano and G. Macino and N. Rajewsky and G. Blandino",

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doi = "10.1186/s13059-017-1368-y [doi]",

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T1 - The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex

AU - Verduci, L.

AU - Ferraiuolo, M.

AU - Sacconi, A.

AU - Ganci, F.

AU - Vitale, J.

AU - Colombo, T.

AU - Paci, P.

AU - Strano, S.

AU - Macino, G.

AU - Rajewsky, N.

AU - Blandino, G.

N1 - LR: 20180103; GR: 14455/AIRC/United States; GR: 13/05/R/42/EPIGEN/United States; JID: 100960660; OTO: NOTNLM; 2017/06/10 00:00 [received]; 2017/12/01 00:00 [accepted]; 2017/12/22 06:00 [entrez]; 2017/12/22 06:00 [pubmed]; 2017/12/22 06:00 [medline]; epublish

PY - 2017/12/20

Y1 - 2017/12/20

N2 - BACKGROUND: Circular RNAs are a class of endogenous RNAs with various functions in eukaryotic cells. Worthy of note, circular RNAs play a critical role in cancer. Currently, nothing is known about their role in head and neck squamous cell carcinoma (HNSCC). The identification of circular RNAs in HNSCC might become useful for diagnostic and therapeutic strategies in HNSCC. RESULTS: Using samples from 115 HNSCC patients, we find that circPVT1 is over-expressed in tumors compared to matched non-tumoral tissues, with particular enrichment in patients with TP53 mutations. circPVT1 up- and down-regulation determine, respectively, an increase and a reduction of the malignant phenotype in HNSCC cell lines. We show that circPVT1 expression is transcriptionally enhanced by the mut-p53/YAP/TEAD complex. circPVT1 acts as an oncogene modulating the expression of miR-497-5p and genes involved in the control of cell proliferation. CONCLUSIONS: This study shows the oncogenic role of circPVT1 in HNSCC, extending current knowledge about the role of circular RNAs in cancer.

AB - BACKGROUND: Circular RNAs are a class of endogenous RNAs with various functions in eukaryotic cells. Worthy of note, circular RNAs play a critical role in cancer. Currently, nothing is known about their role in head and neck squamous cell carcinoma (HNSCC). The identification of circular RNAs in HNSCC might become useful for diagnostic and therapeutic strategies in HNSCC. RESULTS: Using samples from 115 HNSCC patients, we find that circPVT1 is over-expressed in tumors compared to matched non-tumoral tissues, with particular enrichment in patients with TP53 mutations. circPVT1 up- and down-regulation determine, respectively, an increase and a reduction of the malignant phenotype in HNSCC cell lines. We show that circPVT1 expression is transcriptionally enhanced by the mut-p53/YAP/TEAD complex. circPVT1 acts as an oncogene modulating the expression of miR-497-5p and genes involved in the control of cell proliferation. CONCLUSIONS: This study shows the oncogenic role of circPVT1 in HNSCC, extending current knowledge about the role of circular RNAs in cancer.

KW - HNSCC

KW - PVT1

KW - TEAD

KW - YAP

KW - circPVT1

KW - miR-497-5p

KW - mut-p53

U2 - 10.1186/s13059-017-1368-y [doi]

DO - 10.1186/s13059-017-1368-y [doi]

M3 - Article

SN - 1474-7596

VL - 18

SP - 1

EP - 24

JO - Genome Biology

JF - Genome Biology

IS - 1

M1 - 237

ER -

The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex

Abstract

Keywords

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