TY - JOUR
T1 - The neutrophil-activating protein of Helicobacter pylori promotes Th1 immune responses
AU - Amedei, Amedeo
AU - Cappon, Andrea
AU - Codolo, Gaia
AU - Cabrelle, Anna
AU - Polenghi, Alessandra
AU - Benagiano, Marisa
AU - Tasca, Elisabetta
AU - Azzurri, Annalisa
AU - D'Elios, Mario Milco
AU - Prete, Gianfranco Del
AU - De Bernard, Marina
PY - 2006/4/1
Y1 - 2006/4/1
N2 - The Helicobacter pylori neutrophil-activating protein (HP-NAP) is a virulence factor of H. pylori that stimulates in neutrophils high production of oxygen radicals and adhesion to endothelial cells. We report here that HP-NAP is a TLR2 agonist able to induce the expression of IL-12 and IL-23 by neutrophils and monocytes. Addition in culture of HP-NAP, as an immune modulator, to antigen-induced T cell lines resulted in a remarkable increase in the number of IFN-γ-producing T cells and decrease of IL-4-secreting cells, thus shifting the cytokine profile of antigen-activated human T cells from Th2 to a Th1 cytotoxic phenotype. We also found that in vivo HP-NAP elicited an antigen-specific Th1-polarized T cell response in the gastric mucosa of H. pylori-infected patients. These data indicate HP-NAP as an important factor of H. pylori able to elicit cells of the innate immune system to produce IL-12 and IL-23, and they suggest it as a new tool for promoting Th1 immune responses.
AB - The Helicobacter pylori neutrophil-activating protein (HP-NAP) is a virulence factor of H. pylori that stimulates in neutrophils high production of oxygen radicals and adhesion to endothelial cells. We report here that HP-NAP is a TLR2 agonist able to induce the expression of IL-12 and IL-23 by neutrophils and monocytes. Addition in culture of HP-NAP, as an immune modulator, to antigen-induced T cell lines resulted in a remarkable increase in the number of IFN-γ-producing T cells and decrease of IL-4-secreting cells, thus shifting the cytokine profile of antigen-activated human T cells from Th2 to a Th1 cytotoxic phenotype. We also found that in vivo HP-NAP elicited an antigen-specific Th1-polarized T cell response in the gastric mucosa of H. pylori-infected patients. These data indicate HP-NAP as an important factor of H. pylori able to elicit cells of the innate immune system to produce IL-12 and IL-23, and they suggest it as a new tool for promoting Th1 immune responses.
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U2 - 10.1172/JCI27177
DO - 10.1172/JCI27177
M3 - Article
C2 - 16543949
AN - SCOPUS:33645524169
SN - 0021-9738
VL - 116
SP - 1092
EP - 1101
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 4
ER -