TY - JOUR
T1 - The modulation of intracellular glutathione level modulates the mitochondrial response in proliferating rat splenocytes
AU - Pieri, Carlo
AU - Marra, Maurizio
AU - Moroni, Fausto
AU - Marcheselli, Fiorella
AU - Benatti, Cristiana
PY - 1995
Y1 - 1995
N2 - We have investigated the effect of intracellular reduced glutathione (GSH) levels on the mitochondrial parameters of proliferating rat splenocytes. The intracellular GSH content of the cells was altered by continuous culture with buthionine-S, R-sulfoximine (BSO), a specific inhibitor of GSH synthesis. BSO decreased the GSH levels, inhibited DNA synthesis and caused depolarization of mitochondria in 52% of cells stimulated to proliferate for 72 h. These data support the proposition that GSH influences the lymphocyte proliferation at more than one site, one of which is the energy producing machinery.
AB - We have investigated the effect of intracellular reduced glutathione (GSH) levels on the mitochondrial parameters of proliferating rat splenocytes. The intracellular GSH content of the cells was altered by continuous culture with buthionine-S, R-sulfoximine (BSO), a specific inhibitor of GSH synthesis. BSO decreased the GSH levels, inhibited DNA synthesis and caused depolarization of mitochondria in 52% of cells stimulated to proliferate for 72 h. These data support the proposition that GSH influences the lymphocyte proliferation at more than one site, one of which is the energy producing machinery.
KW - Glutathione
KW - Membrane potential
KW - Mitochondria
KW - Splenocytes
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U2 - 10.1016/0167-4943(95)00630-4
DO - 10.1016/0167-4943(95)00630-4
M3 - Article
C2 - 15374208
AN - SCOPUS:0029077134
SN - 0167-4943
VL - 21
SP - 115
EP - 125
JO - Archives of Gerontology and Geriatrics
JF - Archives of Gerontology and Geriatrics
IS - 2
ER -