The first untranslated exon of the human tenascin-C gene plays a regulatory role in gene transcription

Roberto Gherzi; Marco Ponassi; Barbara Gaggero; Luciano Zardi

doi:10.1016/0014-5793(95)00778-8

The first untranslated exon of the human tenascin-C gene plays a regulatory role in gene transcription

Roberto Gherzi, Marco Ponassi, Barbara Gaggero, Luciano Zardi

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

The transcription of the human tenacin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significantly reduce this activation.

Original language	English
Pages (from-to)	335-339
Number of pages	5
Journal	FEBS Letters
Volume	369
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(95)00778-8
Publication status	Published - Aug 7 1995

Keywords

Enhancer
Gene transcription
Silencer
Tenascin-C

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Genetics
Molecular Biology
Structural Biology

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10.1016/0014-5793(95)00778-8

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abstract = "The transcription of the human tenacin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significantly reduce this activation.",

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AU - Zardi, Luciano

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Y1 - 1995/8/7

N2 - The transcription of the human tenacin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significantly reduce this activation.

AB - The transcription of the human tenacin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significantly reduce this activation.

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KW - Gene transcription

KW - Silencer

KW - Tenascin-C

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The first untranslated exon of the human tenascin-C gene plays a regulatory role in gene transcription

Abstract

Keywords

ASJC Scopus subject areas

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