TY - JOUR
T1 - The effect of phosphoramidon and epithelium removal on toluene diisocyanate-induced contractions in guinea-pig bronchi
AU - Mapp, C. E.
AU - Boniotti, A.
AU - Papi, A.
AU - Chitano, P.
AU - Saetta, M.
AU - Di Stefano, A.
AU - Ciaccia, A.
AU - Fabbri, L. M.
PY - 1992
Y1 - 1992
N2 - To evaluate the role of airway neutral endopeptidase 24.11 (NEP) and epithelium removal in the contraction of airway smooth muscle in response to toluene diisocyanate (TDI), we studied the effects of the NEP inhibitor, phosphoramidon, on TDI-induced contractions of guinea-pig bronchial rings with intact epithelium and without epithelium. In preparations with intact epithelium, phosphoramidon (10 μM) potentiated the contractile response to TDI (0.3 mM) mean±SEM, 23.7 ± 2.5% versus 67.9 ± 10.3%, p <0.01). Phosphoramidon also increased TDI-induced contractions in tissues without epithelium (36.9 ± 4.9% versus 52.5 ±7.1%, p <0.05). Removal of the epithelium increased the contractile response to TDI (23.7 ± 2.5% versus 36.9 ± 4.9%, p <0.05). These results demonstrate the response to TDI is increased in epithelium-free compared to intact bronchi and that NEP 24.11 modulates the effects of endogenously released tachykinins by TDI at all of the sites where NEP is found in the airways.
AB - To evaluate the role of airway neutral endopeptidase 24.11 (NEP) and epithelium removal in the contraction of airway smooth muscle in response to toluene diisocyanate (TDI), we studied the effects of the NEP inhibitor, phosphoramidon, on TDI-induced contractions of guinea-pig bronchial rings with intact epithelium and without epithelium. In preparations with intact epithelium, phosphoramidon (10 μM) potentiated the contractile response to TDI (0.3 mM) mean±SEM, 23.7 ± 2.5% versus 67.9 ± 10.3%, p <0.01). Phosphoramidon also increased TDI-induced contractions in tissues without epithelium (36.9 ± 4.9% versus 52.5 ±7.1%, p <0.05). Removal of the epithelium increased the contractile response to TDI (23.7 ± 2.5% versus 36.9 ± 4.9%, p <0.05). These results demonstrate the response to TDI is increased in epithelium-free compared to intact bronchi and that NEP 24.11 modulates the effects of endogenously released tachykinins by TDI at all of the sites where NEP is found in the airways.
KW - Epithelium
KW - Neutral endopeptidase
KW - Toluene diisocyanate
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M3 - Article
C2 - 1315295
AN - SCOPUS:0026593350
SN - 0903-1936
VL - 5
SP - 331
EP - 333
JO - European Journal of Respiratory Diseases
JF - European Journal of Respiratory Diseases
IS - 3
ER -