TY - JOUR
T1 - The effect of dermatan sulfate on in vitro human plasma coagulation, platelet aggregation and βTG/PF4 release
AU - Cofrancesco, E.
AU - Colombi, M.
AU - Gianese, F.
AU - Cortellaro, M.
PY - 1990/2/1
Y1 - 1990/2/1
N2 - We evaluated the in vitro anticoagulant action of dermatan sulfate (DS) (aPTT, antiXa, anti-thrombin) and its effect on human platelet aggregation and βTG/PF4 release induced by threshold doses of aggregating agents, compared with standard heparin (SH). In pooled plasma, DS prolonged aPTT much less than SH, had no measurable antiXa activity, showed an anti-thrombin activity similar to that shown by SH at a tenfold higher dilution. DS had no direct effect on human platelet aggregation and βTG/PF4 release. More-over it did not significantly affect platelet aggregation and release by ADP and collagen, whereas it completely inhibited platelet aggregation and βTG/PF4 release by thrombin. These data in vitro confirm that thrombin inhibition induced by DS is accompained by a far lesser aPTT prolongation compared to heparin, without any appreciable interference with platelet function.
AB - We evaluated the in vitro anticoagulant action of dermatan sulfate (DS) (aPTT, antiXa, anti-thrombin) and its effect on human platelet aggregation and βTG/PF4 release induced by threshold doses of aggregating agents, compared with standard heparin (SH). In pooled plasma, DS prolonged aPTT much less than SH, had no measurable antiXa activity, showed an anti-thrombin activity similar to that shown by SH at a tenfold higher dilution. DS had no direct effect on human platelet aggregation and βTG/PF4 release. More-over it did not significantly affect platelet aggregation and release by ADP and collagen, whereas it completely inhibited platelet aggregation and βTG/PF4 release by thrombin. These data in vitro confirm that thrombin inhibition induced by DS is accompained by a far lesser aPTT prolongation compared to heparin, without any appreciable interference with platelet function.
KW - anticoagulant activity
KW - Dermatan sulfate
KW - platelet aggregation
KW - βTG/PF4 release
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U2 - 10.1016/0049-3848(90)90256-C
DO - 10.1016/0049-3848(90)90256-C
M3 - Article
C2 - 2138364
AN - SCOPUS:0025219105
SN - 0049-3848
VL - 57
SP - 405
EP - 414
JO - Thrombosis Research
JF - Thrombosis Research
IS - 3
ER -