The effect of aphidicolin on Fanconi's anemia lymphocyte chromosomes

B. Porfirio; B. Dallapiccola; E. Gandini

doi:10.1016/0165-7992(85)90061-2

The effect of aphidicolin on Fanconi's anemia lymphocyte chromosomes

B. Porfirio, B. Dallapiccola, E. Gandini

Ospedale Pediatrico Bambino Gesu

Research output: Contribution to journal › Article › peer-review

Abstract

The cytogenetic effect of the DNA polymerase alpha inhibitor aphidicolin (APC) at a dose which did not affect cell cycle progression was determined in normal and Fanconi's anemia (FA) lymphocytes. APC enhanced sister-chromatid exchange (SCE) levels by about twice both in control and FA cells, while the yields of chromosome breakage increased up to 20 times in normal lymphocytes and 4 times in FA cells. APC did not act synergistically with the bifunctional alkylating diepoxybutane in terms of SCE either in normal or in FA lymphocytes. However, chromosome aberrations in cultures from normal subjects were much more than expected by an additive mode of action.

Original language	English
Pages (from-to)	257-263
Number of pages	7
Journal	Mutation Research Letters
Volume	144
Issue number	4
DOIs	https://doi.org/10.1016/0165-7992(85)90061-2
Publication status	Published - 1985

ASJC Scopus subject areas

Genetics
Toxicology
Medicine(all)

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title = "The effect of aphidicolin on Fanconi's anemia lymphocyte chromosomes",

abstract = "The cytogenetic effect of the DNA polymerase alpha inhibitor aphidicolin (APC) at a dose which did not affect cell cycle progression was determined in normal and Fanconi's anemia (FA) lymphocytes. APC enhanced sister-chromatid exchange (SCE) levels by about twice both in control and FA cells, while the yields of chromosome breakage increased up to 20 times in normal lymphocytes and 4 times in FA cells. APC did not act synergistically with the bifunctional alkylating diepoxybutane in terms of SCE either in normal or in FA lymphocytes. However, chromosome aberrations in cultures from normal subjects were much more than expected by an additive mode of action.",

author = "B. Porfirio and B. Dallapiccola and E. Gandini",

year = "1985",

doi = "10.1016/0165-7992(85)90061-2",

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AU - Dallapiccola, B.

AU - Gandini, E.

PY - 1985

Y1 - 1985

N2 - The cytogenetic effect of the DNA polymerase alpha inhibitor aphidicolin (APC) at a dose which did not affect cell cycle progression was determined in normal and Fanconi's anemia (FA) lymphocytes. APC enhanced sister-chromatid exchange (SCE) levels by about twice both in control and FA cells, while the yields of chromosome breakage increased up to 20 times in normal lymphocytes and 4 times in FA cells. APC did not act synergistically with the bifunctional alkylating diepoxybutane in terms of SCE either in normal or in FA lymphocytes. However, chromosome aberrations in cultures from normal subjects were much more than expected by an additive mode of action.

AB - The cytogenetic effect of the DNA polymerase alpha inhibitor aphidicolin (APC) at a dose which did not affect cell cycle progression was determined in normal and Fanconi's anemia (FA) lymphocytes. APC enhanced sister-chromatid exchange (SCE) levels by about twice both in control and FA cells, while the yields of chromosome breakage increased up to 20 times in normal lymphocytes and 4 times in FA cells. APC did not act synergistically with the bifunctional alkylating diepoxybutane in terms of SCE either in normal or in FA lymphocytes. However, chromosome aberrations in cultures from normal subjects were much more than expected by an additive mode of action.

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The effect of aphidicolin on Fanconi's anemia lymphocyte chromosomes

Abstract

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