Abstract
A method for routine measurement of urinary trans,trans-muconic acid. A method is proposed which allows the accurate determination of urinary trans,trans-muconic acid (Ma) by HPLC with UV detection. Sample pretreatment consists of a first purification on strong anionic exchange (SAX) cartridges followed by extraction on "end capped" reversed-phase (C18-EC) ones. Purified samples are directly injected onto a chromatographic column (C18, 150 x 4.6 mm I.D., 3 microns) which is eluted with water: methanol: acetic acid (97:2:1, v/v) mixture followed by a "spike" of acetonitrile. The retention time of MA is 15 min, the coefficient of variation is lower than 3%, the limit of detection is 7 micrograms/l. The method seems suitable for accurate biological monitoring of subjects exposed to benzene.
| Translated title of the contribution | Technical note: proposal of a routine method of accurate measurement of urinary trans,trans-muconic acid |
|---|---|
| Original language | Italian |
| Pages (from-to) | 103-106 |
| Number of pages | 4 |
| Journal | Giornale Italiano di Medicina del Lavoro ed Ergonomia |
| Volume | 20 |
| Issue number | 2 |
| Publication status | Published - 1998 |