Tear proteomics in evaporative dry eye disease

P. Versura; P. Nanni; A. Bavelloni; W. L. Blalock; M. Piazzi; A. Roda; E. C. Campos

doi:10.1038/eye.2010.7

Tear proteomics in evaporative dry eye disease

P. Versura, P. Nanni, A. Bavelloni, W. L. Blalock, M. Piazzi, A. Roda, E. C. Campos

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: To analyze tear protein variations in patients suffering from dry eye symptoms in the presence of tear film instability but without epithelial defects. Methods: Five microlitres of non-stimulated tears from 60 patients, suffering from evaporative dry eye (EDE) with a break-up time (BUT) 10 s) were collected. Tear proteins were separated by mono and bi-dimensional SDS-PAGE electrophoresis and characterized by immunoblotting and enzymatic digestion. Digested peptides were analyzed by liquid chromatography coupled to electrospray ionization quadrupole-time of flight mass spectrometry followed by comparative data analysis into Swiss-Prot human protein database using Mascot. Statistical analysis were performed by applying a t-test for independent data and a Mann-Whitney test for unpaired data (P

Original language	English
Pages (from-to)	1396-1402
Number of pages	7
Journal	Eye (London, England)
Volume	24
Issue number	8
DOIs	https://doi.org/10.1038/eye.2010.7
Publication status	Published - Aug 2010

Keywords

dry eye disease
electrophoresis
human tears
mass spectrometry
proteomics
western-blot

ASJC Scopus subject areas

Ophthalmology

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10.1038/eye.2010.7

Cite this

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abstract = "Purpose: To analyze tear protein variations in patients suffering from dry eye symptoms in the presence of tear film instability but without epithelial defects. Methods: Five microlitres of non-stimulated tears from 60 patients, suffering from evaporative dry eye (EDE) with a break-up time (BUT) 10 s) were collected. Tear proteins were separated by mono and bi-dimensional SDS-PAGE electrophoresis and characterized by immunoblotting and enzymatic digestion. Digested peptides were analyzed by liquid chromatography coupled to electrospray ionization quadrupole-time of flight mass spectrometry followed by comparative data analysis into Swiss-Prot human protein database using Mascot. Statistical analysis were performed by applying a t-test for independent data and a Mann-Whitney test for unpaired data (P",

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AU - Versura, P.

AU - Nanni, P.

AU - Bavelloni, A.

AU - Blalock, W. L.

AU - Piazzi, M.

AU - Roda, A.

AU - Campos, E. C.

PY - 2010/8

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N2 - Purpose: To analyze tear protein variations in patients suffering from dry eye symptoms in the presence of tear film instability but without epithelial defects. Methods: Five microlitres of non-stimulated tears from 60 patients, suffering from evaporative dry eye (EDE) with a break-up time (BUT) 10 s) were collected. Tear proteins were separated by mono and bi-dimensional SDS-PAGE electrophoresis and characterized by immunoblotting and enzymatic digestion. Digested peptides were analyzed by liquid chromatography coupled to electrospray ionization quadrupole-time of flight mass spectrometry followed by comparative data analysis into Swiss-Prot human protein database using Mascot. Statistical analysis were performed by applying a t-test for independent data and a Mann-Whitney test for unpaired data (P

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KW - proteomics

KW - western-blot

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Tear proteomics in evaporative dry eye disease

Abstract

Keywords

ASJC Scopus subject areas

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