Synthesis of fluorescent PMMA-based nanoparticles

Marco Dossi; Raffaele Ferrari; Luca Dragoni; Carlotta Martignoni; Paolo Gaetani; Maurizio D'Incalci; Massimo Morbidelli; Davide Moscatelli

doi:10.1002/mame.201200122

Synthesis of fluorescent PMMA-based nanoparticles

Marco Dossi, Raffaele Ferrari, Luca Dragoni, Carlotta Martignoni, Paolo Gaetani, Maurizio D'Incalci, Massimo Morbidelli, Davide Moscatelli

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

The synthesis of PMMA-based nanoparticles (NPs) covalently labeled with a fluorescent dye is investigated for imaging applications such as cell uptake and biodistribution. Batch emulsion polymerization (BEP) and monomer-starved semi-batch emulsion polymerization (MSSEP) are adopted using SDS. Fluorescent properties are added to these NPs using Rhodamine-B (RhB) as a fluorescent dye covalently bonded to 2-hyroxyethyl-acrylate. The resulting HEMA-RhB monomer is copolymerized with MMA via BEP and MSSEP to synthesize fluorescent NPs. Subsequently, SDS is substituted with a biocompatible surfactant, Tween80, through ionic-exchange resins. ζ-Potential measurements confirmed the complete surfactant exchange that leads to biocompatible fluorescent NPs with tunable size and narrow size distribution.

Original language	English
Pages (from-to)	771-778
Number of pages	8
Journal	Macromolecular Materials and Engineering
Volume	298
Issue number	7
DOIs	https://doi.org/10.1002/mame.201200122
Publication status	Published - Jul 2013

Keywords

biocompatibility
biological applications of polymers
colloids
imaging
nanoparticles

ASJC Scopus subject areas

Organic Chemistry
Materials Chemistry
Polymers and Plastics
Chemical Engineering(all)

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10.1002/mame.201200122

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title = "Synthesis of fluorescent PMMA-based nanoparticles",

abstract = "The synthesis of PMMA-based nanoparticles (NPs) covalently labeled with a fluorescent dye is investigated for imaging applications such as cell uptake and biodistribution. Batch emulsion polymerization (BEP) and monomer-starved semi-batch emulsion polymerization (MSSEP) are adopted using SDS. Fluorescent properties are added to these NPs using Rhodamine-B (RhB) as a fluorescent dye covalently bonded to 2-hyroxyethyl-acrylate. The resulting HEMA-RhB monomer is copolymerized with MMA via BEP and MSSEP to synthesize fluorescent NPs. Subsequently, SDS is substituted with a biocompatible surfactant, Tween80, through ionic-exchange resins. ζ-Potential measurements confirmed the complete surfactant exchange that leads to biocompatible fluorescent NPs with tunable size and narrow size distribution.",

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AU - Dossi, Marco

AU - Ferrari, Raffaele

AU - Dragoni, Luca

AU - Martignoni, Carlotta

AU - Gaetani, Paolo

AU - D'Incalci, Maurizio

AU - Morbidelli, Massimo

AU - Moscatelli, Davide

PY - 2013/7

Y1 - 2013/7

N2 - The synthesis of PMMA-based nanoparticles (NPs) covalently labeled with a fluorescent dye is investigated for imaging applications such as cell uptake and biodistribution. Batch emulsion polymerization (BEP) and monomer-starved semi-batch emulsion polymerization (MSSEP) are adopted using SDS. Fluorescent properties are added to these NPs using Rhodamine-B (RhB) as a fluorescent dye covalently bonded to 2-hyroxyethyl-acrylate. The resulting HEMA-RhB monomer is copolymerized with MMA via BEP and MSSEP to synthesize fluorescent NPs. Subsequently, SDS is substituted with a biocompatible surfactant, Tween80, through ionic-exchange resins. ζ-Potential measurements confirmed the complete surfactant exchange that leads to biocompatible fluorescent NPs with tunable size and narrow size distribution.

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KW - biological applications of polymers

KW - colloids

KW - imaging

KW - nanoparticles

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Synthesis of fluorescent PMMA-based nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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