Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: A pilot study

P. Agostoni; C. Banfi; M. Brioschi; D. Magrì; S. Sciomer; G. Berna; C. Brambillasca; G. Marenzi; E. Sisillo

doi:10.1183/09031936.00045910

Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: A pilot study

P. Agostoni, C. Banfi, M. Brioschi, D. Magrì, S. Sciomer, G. Berna, C. Brambillasca, G. Marenzi, E. Sisillo

Centro Cardiologico Monzino

Research output: Contribution to journal › Article › peer-review

Abstract

Surfactant derived protein B (SPB) and plasma receptor for advanced glycation end products (RAGE) have been proposed as markers of lung injury. The former is produced specifically by pneumocytes while RAGE production is present in several body tissues. Cardiopulmonary bypass (CPB) generates a transient lung injury. We measured SPB and RAGE in plasma before surgery and after CPB, as well as 24 h and 48 h later. We analysed plasma samples from 20 subjects scheduled for elective coronary artery bypass grafting. We performed a quantitative analysis of plasma levels of RAGE and SPB mature form (8 kDa) by ELISA and a semi-quantitative analysis of SPB immature form (∼40 kDa) by Western blotting. Surgery procedures were uneventful. After CPB RAGE median (75th-25th interquartile difference) increased from 633 (539) pg·mL ^-1 to 1,362 (557) pg·mL^-1 (p-1 to 20,307 (19,873) ng·mL^-1 (p

Original language	English
Pages (from-to)	841-847
Number of pages	7
Journal	European Respiratory Journal
Volume	37
Issue number	4
DOIs	https://doi.org/10.1183/09031936.00045910
Publication status	Published - Apr 1 2011

Keywords

Gas diffusion
Heart failure
Lung injury
Surfactant

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

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10.1183/09031936.00045910

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abstract = "Surfactant derived protein B (SPB) and plasma receptor for advanced glycation end products (RAGE) have been proposed as markers of lung injury. The former is produced specifically by pneumocytes while RAGE production is present in several body tissues. Cardiopulmonary bypass (CPB) generates a transient lung injury. We measured SPB and RAGE in plasma before surgery and after CPB, as well as 24 h and 48 h later. We analysed plasma samples from 20 subjects scheduled for elective coronary artery bypass grafting. We performed a quantitative analysis of plasma levels of RAGE and SPB mature form (8 kDa) by ELISA and a semi-quantitative analysis of SPB immature form (∼40 kDa) by Western blotting. Surgery procedures were uneventful. After CPB RAGE median (75th-25th interquartile difference) increased from 633 (539) pg·mL -1 to 1,362 (557) pg·mL-1 (p-1 to 20,307 (19,873) ng·mL-1 (p",

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AU - Agostoni, P.

AU - Banfi, C.

AU - Brioschi, M.

AU - Magrì, D.

AU - Sciomer, S.

AU - Berna, G.

AU - Brambillasca, C.

AU - Marenzi, G.

AU - Sisillo, E.

PY - 2011/4/1

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N2 - Surfactant derived protein B (SPB) and plasma receptor for advanced glycation end products (RAGE) have been proposed as markers of lung injury. The former is produced specifically by pneumocytes while RAGE production is present in several body tissues. Cardiopulmonary bypass (CPB) generates a transient lung injury. We measured SPB and RAGE in plasma before surgery and after CPB, as well as 24 h and 48 h later. We analysed plasma samples from 20 subjects scheduled for elective coronary artery bypass grafting. We performed a quantitative analysis of plasma levels of RAGE and SPB mature form (8 kDa) by ELISA and a semi-quantitative analysis of SPB immature form (∼40 kDa) by Western blotting. Surgery procedures were uneventful. After CPB RAGE median (75th-25th interquartile difference) increased from 633 (539) pg·mL -1 to 1,362 (557) pg·mL-1 (p-1 to 20,307 (19,873) ng·mL-1 (p

AB - Surfactant derived protein B (SPB) and plasma receptor for advanced glycation end products (RAGE) have been proposed as markers of lung injury. The former is produced specifically by pneumocytes while RAGE production is present in several body tissues. Cardiopulmonary bypass (CPB) generates a transient lung injury. We measured SPB and RAGE in plasma before surgery and after CPB, as well as 24 h and 48 h later. We analysed plasma samples from 20 subjects scheduled for elective coronary artery bypass grafting. We performed a quantitative analysis of plasma levels of RAGE and SPB mature form (8 kDa) by ELISA and a semi-quantitative analysis of SPB immature form (∼40 kDa) by Western blotting. Surgery procedures were uneventful. After CPB RAGE median (75th-25th interquartile difference) increased from 633 (539) pg·mL -1 to 1,362 (557) pg·mL-1 (p-1 to 20,307 (19,873) ng·mL-1 (p

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Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: A pilot study

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Keywords

ASJC Scopus subject areas

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