Surface distribution and internalization of erbB-2 Proteins

Lavinia Vittoria Lotti, Claudia Di Lazzaro, Claudia Zompetta, Luigi Frati, Maria Rosaria Torrisi

Research output: Contribution to journalArticlepeer-review


We report the localization over the cell surface and the early steps of antibody-induced internalization of the product of the erbB-2 proto-oncogene, structurally related to the epidermal growth factor receptor (EGFR). We show that erbB-2/p185 is mostly excluded from endocytic pits on the cell surface. Incubation at 37 °C with an anti-erbB-2/p185 monoclonal antibody induces the rapid entry of the protein into the cell. Similar internalization is shown by a chimeric molecule EGFB/erbB-2 in response to EGF. Both the timing and the pathway of internalization followed by the erbB-2/ p185 appear totally similar to those described for the EGFR. At variance with the normal erbB-2/p185, two mutant activated erbB-2 proteins are frequently localized within endocytic pits of the cell surface, indicating that mutations in the transmembrane regions may determine constitutive internalization of the protein.

Original languageEnglish
Pages (from-to)274-280
Number of pages7
JournalExperimental Cell Research
Issue number2
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Cell Biology


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