Stable changes in CD4 + T lymphocyte miRNA expression after exposure to HIV-1

Fabio Bignami, Elisabetta Pilotti, Linda Bertoncelli, Paola Ronzi, Mariolina Gulli, Nelson Marmiroli, Giacomo Magnani, Marcello Pinti, Lucia Lopalco, Cristina Mussini, Roberta Ruotolo, Massimo Galli, Andrea Cossarizza, Claudio Casoli

Research output: Contribution to journalArticlepeer-review


MicroRNAs (miRNAs) inhibit HIV-1 expression by either modulating host innate immunity or by directly interfering with viral mRNAs. We evaluated the expression of 377 miRNAs in CD4 + T cells from HIV-1 élite long-term nonprogressors (éLTNPs), naive patients, and multiply exposed uninfected (MEU) patients, and we observed that the éLTNP patients clustered with naive patients, whereas all MEU subjects grouped together. The discriminatory power of miRNAs showed that 21 miRNAs significantly differentiated éLTNP from MEU patients and 23 miRNAs distinguished naive from MEU patients, whereas only 1 miRNA (miR-155) discriminated éLTNP from naive patients. We proposed that miRNA expression may discriminate between HIV-1-infected and -exposed but negative patients. Analysis of miRNAs expression after exposure of healthy CD4 +T cells to gp120 in vitro confirmed our hypothesis that a miRNA profile could be the result not only of a productive infection but also of the exposure to HIV-1 products that leave a signature in immune cells. The comparison of normalized Dicer and Drosha expression in ex vivo and in vitro condition revealed that these enzymes did not affect the change of miRNA profiles, supporting the existence of a Dicer-independent biogenesis pathway.

Original languageEnglish
Pages (from-to)6259-6267
Number of pages9
Issue number26
Publication statusPublished - Jun 28 2012

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology


Dive into the research topics of 'Stable changes in CD4 + T lymphocyte miRNA expression after exposure to HIV-1'. Together they form a unique fingerprint.

Cite this