TY - JOUR
T1 - Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins
T2 - A comparative study
AU - Ascenzi, Paolo
AU - Coletta, Massimo
AU - Desideri, Alessandro
AU - Petruzzelli, Raffaele
AU - Polizio, Francesca
AU - Bolognesi, Martino
AU - Condò, Saverio Giovanni
AU - Giardina, Bruno
PY - 1992
Y1 - 1992
N2 - The spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous man, horse, buffalo, deer, mouflon, musk ox, ox, and reindeer hemoglobin (HbNO) have been investigated in the absence of any allosteric effector at pH 6.5 (in 0.1 M 2-[N-morpholino]ethanesulphonic acid/NaOH chloride-free buffer system), as well as at 100 K and/or 20°C. Man and horse HbNO show spectroscopic properties that are generally taken as typical of the high affinity state of ferrous tetrameric Hb's; on the other hand, the spectroscopic properties of ruminant (i.e., buffalo, deer, mouflon, musk ox, ox, and reindeer) HbNO are characteristic of the low affinity conformation. These results are in keeping with the functional properties of the mammalian Hb's considered and have been related to the peculiar low oxygen affinity of ruminant Hb's.
AB - The spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous man, horse, buffalo, deer, mouflon, musk ox, ox, and reindeer hemoglobin (HbNO) have been investigated in the absence of any allosteric effector at pH 6.5 (in 0.1 M 2-[N-morpholino]ethanesulphonic acid/NaOH chloride-free buffer system), as well as at 100 K and/or 20°C. Man and horse HbNO show spectroscopic properties that are generally taken as typical of the high affinity state of ferrous tetrameric Hb's; on the other hand, the spectroscopic properties of ruminant (i.e., buffalo, deer, mouflon, musk ox, ox, and reindeer) HbNO are characteristic of the low affinity conformation. These results are in keeping with the functional properties of the mammalian Hb's considered and have been related to the peculiar low oxygen affinity of ruminant Hb's.
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U2 - 10.1016/0162-0134(92)84038-O
DO - 10.1016/0162-0134(92)84038-O
M3 - Article
C2 - 1316949
AN - SCOPUS:0026567546
SN - 0162-0134
VL - 45
SP - 31
EP - 37
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
IS - 1
ER -