TY - JOUR
T1 - Specific role for protein kinase Cα in the constitutive and regulated secretion of amyloid precursor protein in human skin fibroblasts
AU - Benussi, Luisa
AU - Govoni, Stefano
AU - Gasparini, Laura
AU - Binetti, Giuliano
AU - Trabucchi, Marco
AU - Bianchetti, Angelo
AU - Racchi, Marco
PY - 1998/1/9
Y1 - 1998/1/9
N2 - Reduced levels of protein kinase Cα (PKCα) seems to be related to an altered amyloid precursor protein (APP) secretion in fibroblasts from Alzheimer's disease (AD) patients. In this report we used a specific inhibitor of PKCα (Go-6976), to investigate the role of PKCα in the basal and phorbol esters regulated secretion of soluble APP (sAPP) in human fibroblasts derived from healthy aged volunteers. Treatment with Go-6976 alone reduced basal secretion by a maximum of 39%, compared to untreated cells, suggesting the partial dependence of constitutive APP secretory pathway on PKCα enzyme. Moreover Go-6976 treatment completely abolished the effect of phorbol-esters mediated PKC stimulation on sAPP release, suggesting that PKCα is the only PKC isoform involved in controlling the secretion of sAPP in human fibroblasts.
AB - Reduced levels of protein kinase Cα (PKCα) seems to be related to an altered amyloid precursor protein (APP) secretion in fibroblasts from Alzheimer's disease (AD) patients. In this report we used a specific inhibitor of PKCα (Go-6976), to investigate the role of PKCα in the basal and phorbol esters regulated secretion of soluble APP (sAPP) in human fibroblasts derived from healthy aged volunteers. Treatment with Go-6976 alone reduced basal secretion by a maximum of 39%, compared to untreated cells, suggesting the partial dependence of constitutive APP secretory pathway on PKCα enzyme. Moreover Go-6976 treatment completely abolished the effect of phorbol-esters mediated PKC stimulation on sAPP release, suggesting that PKCα is the only PKC isoform involved in controlling the secretion of sAPP in human fibroblasts.
KW - Alzheimer's disease
KW - Amyloid precursor protein
KW - Human skin fibroblasts
KW - Indolocarbazole Go-6976
KW - Phorbol esters
KW - Protein kinase C
KW - Staurosporine
UR - http://www.scopus.com/inward/record.url?scp=0032498135&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032498135&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(97)00894-X
DO - 10.1016/S0304-3940(97)00894-X
M3 - Article
C2 - 9486481
AN - SCOPUS:0032498135
SN - 0304-3940
VL - 240
SP - 97
EP - 101
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -