Simultaneous staining of cytoplasmic iron and collagen matrix in human liver biopsy specimens

Fabio Grizzi, G. Ceva-Grimaldi, B. Franceschini, M. Roncalli, M. Chiriva-Internati, N. Dioguardi

Research output: Contribution to journalArticlepeer-review


One of the major liver fibrogenic activators is the cellular iron overload that can severely damage parenchymal and non-parenchymal cells. The aim of this study was to investigate a histochemical staining technique that allows the simultaneous detection of the irregular deposition of matrix collagen and both the amount and distribution of iron in liver cells on the same histological section. The method was evaluated using 3-μm histological sections obtained from ten standard liver biopsy specimens taken from patients with hepatitis C virus-related diseases and simultaneous liver cell iron overload. The results indicate that the double-staining technique is simple, sensitive and rapid, and can be routinely applied to liver biopsy specimens for diagnostic purposes. Furthermore, it may also facilitate the study of the complex relationship between hepatic fibrosis and iron overload during common genetic or secondary liver metabolic disorders.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalEuropean journal of histochemistry : EJH
Issue number1
Publication statusPublished - 2002


  • Collagen
  • Extra-cellular matrix
  • Histochemistry
  • Iron
  • Liver

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Animal Science and Zoology
  • Developmental Biology


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