Simian-virus-40 footprints in human lymphoproliferative disorders of HIV- and HIV+ patients

Fernanda Martini, Riccardo Dolcetti, Annunziata Gloghini, Laura Iaccheri, Antonino Carbone, Mauro Boiocchi, Mauro Tognon

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SV40 sequences were investigated by PCR DNA amplification followed by filter hybridization in a series of human lymphoproliferative disorders obtained from human-immunodeficiency-virus (HIV)-seronegative and HIV- infected patients. Our PCR and filter-hybridization conditions enabled us to detect SV40 sequences in the range of 10-4 to 10-2 genome equivalents per cell. In non-Hodgkin's lymphomas (NHL) from HIV- patients, SV40 footprints were found in 11 out of 79 (13.9%) samples, while in NHL from HIV+ patients SV40 DNA sequences were detected in 2/16 (12.5%). In Hodgkin's disease (HD), SV40 sequences were found in 7/43 (16.3%) and 1/12 (8.3%) in HIV- and HIV+ patients respectively. A slightly higher prevalence of SV40 footprints was observed in reactive lympho-adenopathies both in HIV- (3/9, 33.3%) and in HIV+ (6/17, 35.3%) patients. Sequence analysis of 2 NHL and 2 HD DNA samples established that the amplified PCR products belong to the SV40 sequences. SV40 prevalence and load were similar in samples from HIV-seronegative and HIV-infected individuals, suggesting that SV40 probably does not undergo strong reactivation phenomena in the context of HIV-related immunosuppression. Moreover, the large T-antigen(Tag) expression was detected by immunohistochemistry in 5/18 SV40-DNA-positive samples analyzed; however, few tumor cells (

Original languageEnglish
Pages (from-to)669-674
Number of pages6
JournalInternational Journal of Cancer
Issue number6
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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