TY - JOUR
T1 - Sex differences in GABAAergic system in rat substantia nigra pars reticulata
AU - Ravizza, Teresa
AU - Friedman, Linda K.
AU - Moshé, Solomon L.
AU - Velíšková, Jana
PY - 2003/8
Y1 - 2003/8
N2 - The substantia nigra pars reticulata (SNR) is involved in the control of movement disorders including seizures through its GABAergic neurons. Microinfusions of muscimol (a GABAA receptor agonist) produce specific effects on seizures depending on sex, infusion site (SNRanterior or SNRposterior) and age. To assess whether these effects are due to sex differences in GABAergic indices within the SNR we analyzed the expression of α1 subunit mRNA of the GABAA receptor and the levels of GABA immunoreactivity (IR) of male and female rats at postnatal day 15 (PN15) and PN30. In each age, within the same SNR region, expression of α1 subunit mRNA and intensity of GABA IR per neuron was higher in females compared to males. At PN15, in both sexes, there were no regional differences in expression of α1 subunit mRNA and intensity of GABA IR. However, at PN30 in both sexes, expression of α1 subunit mRNA and intensity of GABA IR per cell was higher in SNRanterior than in SNRposterior. These results demonstrate that expression of α1 subunit mRNA for GABAA receptor and levels of GABA IR in the SNR are sex- and site-specific, which may contribute to sex-, regional- and age-related differences in the expression of movement disorders and seizures.
AB - The substantia nigra pars reticulata (SNR) is involved in the control of movement disorders including seizures through its GABAergic neurons. Microinfusions of muscimol (a GABAA receptor agonist) produce specific effects on seizures depending on sex, infusion site (SNRanterior or SNRposterior) and age. To assess whether these effects are due to sex differences in GABAergic indices within the SNR we analyzed the expression of α1 subunit mRNA of the GABAA receptor and the levels of GABA immunoreactivity (IR) of male and female rats at postnatal day 15 (PN15) and PN30. In each age, within the same SNR region, expression of α1 subunit mRNA and intensity of GABA IR per neuron was higher in females compared to males. At PN15, in both sexes, there were no regional differences in expression of α1 subunit mRNA and intensity of GABA IR. However, at PN30 in both sexes, expression of α1 subunit mRNA and intensity of GABA IR per cell was higher in SNRanterior than in SNRposterior. These results demonstrate that expression of α1 subunit mRNA for GABAA receptor and levels of GABA IR in the SNR are sex- and site-specific, which may contribute to sex-, regional- and age-related differences in the expression of movement disorders and seizures.
KW - α Subunit
KW - γ-Aminobutyric acid
KW - Midbrain
KW - Sexual dimorphism
UR - http://www.scopus.com/inward/record.url?scp=0038500753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038500753&partnerID=8YFLogxK
U2 - 10.1016/S0736-5748(03)00069-8
DO - 10.1016/S0736-5748(03)00069-8
M3 - Article
C2 - 12850057
AN - SCOPUS:0038500753
SN - 0736-5748
VL - 21
SP - 245
EP - 254
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
IS - 5
ER -