TY - JOUR
T1 - Separation of the valence intermediates of human haemoglobin by high-performance chromatofocusing
AU - Bolzacchini, Ezio
AU - Fermo, Isabella
AU - Rovida, Ermanna
AU - Colombo, Roberto
AU - Samaja, Michele
PY - 1987/6/26
Y1 - 1987/6/26
N2 - Investigations into the properties of haemoglobin often require the isolation of the valence intermediates (αo)2β+)2 and (α+ βo
2)2. Chromatofocusing with an anion-exchange gel (Mono PTM; Pharmacia, particle size 10 μm) in an HR5/20 column at various temperatures (10-25°C) provides an excellent method for this task. A linearly decreasing pH gradient (8 to 7, generated by Polybuffer 96, Pharmacia) eluted sequentially the species methaemoglobin, (αo
2β+)2, (α+ βo
2)2 and oxygenated haemoglobin. Calibration graphs help in quantitative analyses. This method is simpler and less time consuming and provides a similar or even better resolution than the traditional ion-exchange or isoelectric focusing methods.
AB - Investigations into the properties of haemoglobin often require the isolation of the valence intermediates (αo)2β+)2 and (α+ βo
2)2. Chromatofocusing with an anion-exchange gel (Mono PTM; Pharmacia, particle size 10 μm) in an HR5/20 column at various temperatures (10-25°C) provides an excellent method for this task. A linearly decreasing pH gradient (8 to 7, generated by Polybuffer 96, Pharmacia) eluted sequentially the species methaemoglobin, (αo
2β+)2, (α+ βo
2)2 and oxygenated haemoglobin. Calibration graphs help in quantitative analyses. This method is simpler and less time consuming and provides a similar or even better resolution than the traditional ion-exchange or isoelectric focusing methods.
UR - http://www.scopus.com/inward/record.url?scp=0023162480&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023162480&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)85006-8
DO - 10.1016/S0021-9673(01)85006-8
M3 - Article
C2 - 3654817
AN - SCOPUS:0023162480
SN - 0021-9673
VL - 397
SP - 233
EP - 237
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -