Self γ2ab Protein Is Presented in Vivo by γ2ab B Cells but Not by Dendritic Cells

Francesca Granucci, Maria Foti, Andrea Cossarizza, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticlepeer-review


We have previously shown that IgG2ab Ig does not induce tolerance in MHC class II restricted CD4 T cells in a TCR transgenic model and that anti-IgC2ab transgenic T cells specific for peptide 435-451 are indeed present in the periphery where they interact with γ2ab-positive B cells. We also observed that because T cell tolerance depends on the presentation of self peptides, it was probable that IgG2ab was not easily processed and presented in vivo. In this study, we have investigated the presentation of naturally processed γ2ab (435-451) determinants to specific T cells. Dendritic cells, macrophages, and B cells were purified from the spleens of Igh-1b mice. These cells were then functionally tested for the presence of specific peptide-MHC complexes. The results showed that, in vivo, γ2ab-producing B cells, but not dendritic cells, are the only APCs able to present this self peptide. This indicates that recognition of the IgG2ab-self peptide is exclusively mediated by T-B cell interaction.

Original languageEnglish
Pages (from-to)1794-1799
Number of pages6
JournalJournal of Immunology
Issue number4
Publication statusPublished - Aug 15 1997

ASJC Scopus subject areas

  • Immunology


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