Secretion of cytokines upon allogeneic stimulation: Effect of aging

M. Molteni, S. Della Bella, B. Mascagni, C. Coppola, V. De Micheli, C. Zulian, S. Birindelli, M. Vanoli, R. Scorza

Research output: Contribution to journalArticlepeer-review


The aim of the present study was to investigate the in vitro mitotic response and cytokine production after allogeneic stimulation of peripheral blood mononuclear cells (PBMC) from healthy elderly subjects. Interleukin-1 (IL-1), interleukin-2 (IL-2), gamma-Interferon (gamma-IFN), and Tumor Necrosis Factor alpha (TNF-alpha) were detected in the supernatants of mixed lymphocyte cultures (MLC). The in vitro proliferative response was significantly reduced in the elderly subjects. The amount of IL-2 detected in the supernatant from cultures of cells from elderly donors was higher than for young controls, as were the production of cytokines predominantly secreted by the macrophage population (IL-1 and TNF-alpha). There was no difference in the production of gamma-IFN by cells of elderly and young subjects.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalJournal of Biological Regulators and Homeostatic Agents
Issue number2
Publication statusPublished - 1994


  • aging MLC
  • gamma-IFN
  • IL-1
  • IL-2
  • TNF-alpha

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Endocrinology
  • Physiology
  • Immunology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)


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