TY - JOUR
T1 - Relationships between paraoxon and 2-coumaranone hydrolytic activities in sera genotyped for PON1 Q192R polymorphism
AU - Giacominelli-Stuffler, Roberto
AU - Angelucci, Clotilde Beatrice
AU - Liberatoscioli, Laura
AU - Cortese, Claudio
AU - Dainese, Enrico
AU - Maccarrone, Mauro
PY - 2009/10
Y1 - 2009/10
N2 - Objectives: To set-up a method for a direct evaluation in human serum of paraoxonase enzymatic activities, establishing a possible correlation with Q192R genotype polymorphism. Design and methods: 101 different human serum samples were genotyped for paraoxonase Q192R polymorphism by PCR restriction analysis, and evaluated spectrophotometrically with regard to paraoxon and 2-coumaranone hydrolytic activities. Both activities of paraoxonase were assayed, quantified through normalization by arylesterase activity, and compared with the data concerning Q/R genetic polymorphism. Results: The mean normalized paraoxonase activity was found to be significantly higher in RR than in QQ human sera (3.99 ± 0.6 versus 1.32 ± 0.44; P <0.0001); instead, the 2-coumaranone hydrolysis showed an opposite trend (0.10 ± 0.02 versus 0.23 ± 0.04, in RR and QQ sera respectively; P <0.0001). Conclusions: These methods were successfully applied to the whole serum, suggesting a possible use of this approach for a clinically relevant phenotypic characterization.
AB - Objectives: To set-up a method for a direct evaluation in human serum of paraoxonase enzymatic activities, establishing a possible correlation with Q192R genotype polymorphism. Design and methods: 101 different human serum samples were genotyped for paraoxonase Q192R polymorphism by PCR restriction analysis, and evaluated spectrophotometrically with regard to paraoxon and 2-coumaranone hydrolytic activities. Both activities of paraoxonase were assayed, quantified through normalization by arylesterase activity, and compared with the data concerning Q/R genetic polymorphism. Results: The mean normalized paraoxonase activity was found to be significantly higher in RR than in QQ human sera (3.99 ± 0.6 versus 1.32 ± 0.44; P <0.0001); instead, the 2-coumaranone hydrolysis showed an opposite trend (0.10 ± 0.02 versus 0.23 ± 0.04, in RR and QQ sera respectively; P <0.0001). Conclusions: These methods were successfully applied to the whole serum, suggesting a possible use of this approach for a clinically relevant phenotypic characterization.
KW - Enzymatic activities
KW - Genetic polymorphism
KW - High density lipoproteins
KW - Paraoxonase
KW - PON1
UR - http://www.scopus.com/inward/record.url?scp=70249125018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70249125018&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2009.07.006
DO - 10.1016/j.clinbiochem.2009.07.006
M3 - Article
C2 - 19615988
AN - SCOPUS:70249125018
SN - 0009-9120
VL - 42
SP - 1512
EP - 1516
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 15
ER -