TY - JOUR
T1 - Recombinant human antibodies specific for hepatitis C virus proteins
AU - Esposito, G.
AU - Morea, V.
AU - Scarselli, E.
AU - Cerino, A.
AU - Mondelli, M. U.
AU - La Monica, N.
AU - Traboni, C.
PY - 1997
Y1 - 1997
N2 - Human antibodies to hepatitis C virus core, NS4A and NS3 were cloned in a prokaryotic vector and expressed as soluble Fab fragments and as phage-displayed Fabs. The recombinant Fabs were shown to be a suitable tool for immunohistochemistry, since they recognize the cognate antigen expressed in mammalian cells. The nucleotide sequence of the cDNA for the variable domains of these antibodies was determined and the V-gene usage was derived. On the basis of the deduced amino acid sequence, a structural model of the V domains of the Fabs was constructed.
AB - Human antibodies to hepatitis C virus core, NS4A and NS3 were cloned in a prokaryotic vector and expressed as soluble Fab fragments and as phage-displayed Fabs. The recombinant Fabs were shown to be a suitable tool for immunohistochemistry, since they recognize the cognate antigen expressed in mammalian cells. The nucleotide sequence of the cDNA for the variable domains of these antibodies was determined and the V-gene usage was derived. On the basis of the deduced amino acid sequence, a structural model of the V domains of the Fabs was constructed.
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U2 - 10.1007/s007050050106
DO - 10.1007/s007050050106
M3 - Article
C2 - 9349306
AN - SCOPUS:0030999033
SN - 0304-8608
VL - 142
SP - 601
EP - 610
JO - Archives of Virology
JF - Archives of Virology
IS - 3
ER -