Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags

Jilin Tang; Andreas Ebner; Helga Badelt-Lichtblau; Christine Völlenkle; Christian Rankl; Bernhard Kraxberger; Michael Leitner; Linda Wildling; Hermann J. Gruber; Uwe B. Sleytr; Nicola Ilk; Peter Hinterdorfer

doi:10.1021/nl802092c

Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags

Jilin Tang, Andreas Ebner, Helga Badelt-Lichtblau, Christine Völlenkle, Christian Rankl, Bernhard Kraxberger, Michael Leitner, Linda Wildling, Hermann J. Gruber, Uwe B. Sleytr, Nicola Ilk, Peter Hinterdorfer

IRCCS Policlinico San Donato

Research output: Contribution to journal › Article › peer-review

Abstract

Functional nanoarrays were fabricated using the chimeric bacterial cell surface layer (S-layer) protein rSbpA fused with the affinity tag Strep-tagll and characterized using various atomic force microscopy (AFM) techniques in aqueous environment. The accessibility of Strep-tagll was verified by single-molecule force spectroscopy studies employing Strep-Tactin as specific ligand. Simultaneous topography and recognition imaging (TREC) of the nanoarray yielded high resolution maps of the Strep-tagll binding sites with a positional accuracy of 1.5 nm. The nanoarrays were used as template for constructing highly ordered molecular binding blocks.

Original language	English
Pages (from-to)	4312-4319
Number of pages	8
Journal	Nano Letters
Volume	8
Issue number	12
DOIs	https://doi.org/10.1021/nl802092c
Publication status	Published - Dec 2008

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Mechanical Engineering
Materials Science(all)
Condensed Matter Physics

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10.1021/nl802092c

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title = "Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags",

abstract = "Functional nanoarrays were fabricated using the chimeric bacterial cell surface layer (S-layer) protein rSbpA fused with the affinity tag Strep-tagll and characterized using various atomic force microscopy (AFM) techniques in aqueous environment. The accessibility of Strep-tagll was verified by single-molecule force spectroscopy studies employing Strep-Tactin as specific ligand. Simultaneous topography and recognition imaging (TREC) of the nanoarray yielded high resolution maps of the Strep-tagll binding sites with a positional accuracy of 1.5 nm. The nanoarrays were used as template for constructing highly ordered molecular binding blocks.",

author = "Jilin Tang and Andreas Ebner and Helga Badelt-Lichtblau and Christine V{\"o}llenkle and Christian Rankl and Bernhard Kraxberger and Michael Leitner and Linda Wildling and Gruber, {Hermann J.} and Sleytr, {Uwe B.} and Nicola Ilk and Peter Hinterdorfer",

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doi = "10.1021/nl802092c",

language = "English",

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T1 - Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags

AU - Tang, Jilin

AU - Ebner, Andreas

AU - Badelt-Lichtblau, Helga

AU - Völlenkle, Christine

AU - Rankl, Christian

AU - Kraxberger, Bernhard

AU - Leitner, Michael

AU - Wildling, Linda

AU - Gruber, Hermann J.

AU - Sleytr, Uwe B.

AU - Ilk, Nicola

AU - Hinterdorfer, Peter

PY - 2008/12

Y1 - 2008/12

N2 - Functional nanoarrays were fabricated using the chimeric bacterial cell surface layer (S-layer) protein rSbpA fused with the affinity tag Strep-tagll and characterized using various atomic force microscopy (AFM) techniques in aqueous environment. The accessibility of Strep-tagll was verified by single-molecule force spectroscopy studies employing Strep-Tactin as specific ligand. Simultaneous topography and recognition imaging (TREC) of the nanoarray yielded high resolution maps of the Strep-tagll binding sites with a positional accuracy of 1.5 nm. The nanoarrays were used as template for constructing highly ordered molecular binding blocks.

AB - Functional nanoarrays were fabricated using the chimeric bacterial cell surface layer (S-layer) protein rSbpA fused with the affinity tag Strep-tagll and characterized using various atomic force microscopy (AFM) techniques in aqueous environment. The accessibility of Strep-tagll was verified by single-molecule force spectroscopy studies employing Strep-Tactin as specific ligand. Simultaneous topography and recognition imaging (TREC) of the nanoarray yielded high resolution maps of the Strep-tagll binding sites with a positional accuracy of 1.5 nm. The nanoarrays were used as template for constructing highly ordered molecular binding blocks.

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Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags

Abstract

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