Quantitative determination of methemoglobin by measuring the solvent-water proton-nuclear magnetic resonance relaxation rate

Silvio Aime; Walter Dastrú; Mauro Fasano; Aldo Arnelli; Massimo Castagnola; Bruno Giardina; Paolo Ascenzi

Quantitative determination of methemoglobin by measuring the solvent-water proton-nuclear magnetic resonance relaxation rate

Silvio Aime, Walter Dastrú, Mauro Fasano, Aldo Arnelli, Massimo Castagnola, Bruno Giardina, Paolo Ascenzi

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

We report a new method for the quantitative determination of human methemoglobin (metHb) based on the Measurement of the solvent-water proton-nuclear magnetic resonance (NMR) relaxation rate R₁ [normalized to 1 mmol/L hemoglobin (Hb) concentration]. MetHb (%) is estimated from the linear dependence of R₁ on the metHb concentration, taking into account the simple relationship [MetHb] = [(R₁- R₁ ^HbO2)/(R₁ ^metHb - R₁ ^HbO2)] ·100, where RR₁ ^HbO2 and R₁ ^metHb are values for the solvent-water relaxation rate of standard 1.0 mmol/L solutions of the oxygenated derivative of human hemoglobin (HbO₂) and of metHb, respectively. The minimum metHb that may be determined from the analysis of the experimental data is 0.5 ± 0.4%.

Original language	English
Pages (from-to)	2401-2404
Number of pages	4
Journal	Clinical Chemistry
Volume	38
Issue number	12
Publication status	Published - 1992

Keywords

Hemoglobin
Intermethod comparison

ASJC Scopus subject areas

Clinical Biochemistry

Cite this

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title = "Quantitative determination of methemoglobin by measuring the solvent-water proton-nuclear magnetic resonance relaxation rate",

abstract = "We report a new method for the quantitative determination of human methemoglobin (metHb) based on the Measurement of the solvent-water proton-nuclear magnetic resonance (NMR) relaxation rate R1 [normalized to 1 mmol/L hemoglobin (Hb) concentration]. MetHb (%) is estimated from the linear dependence of R1 on the metHb concentration, taking into account the simple relationship [MetHb] = [(R1- R1 HbO2)/(R1 metHb - R1 HbO2)] ·100, where RR1 HbO2 and R1 metHb are values for the solvent-water relaxation rate of standard 1.0 mmol/L solutions of the oxygenated derivative of human hemoglobin (HbO2) and of metHb, respectively. The minimum metHb that may be determined from the analysis of the experimental data is 0.5 ± 0.4%.",

keywords = "Hemoglobin, Intermethod comparison",

author = "Silvio Aime and Walter Dastr{\'u} and Mauro Fasano and Aldo Arnelli and Massimo Castagnola and Bruno Giardina and Paolo Ascenzi",

year = "1992",

language = "English",

volume = "38",

pages = "2401--2404",

journal = "Clinical Chemistry",

issn = "0009-9147",

publisher = "American Association for Clinical Chemistry Inc.",

number = "12",

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TY - JOUR

T1 - Quantitative determination of methemoglobin by measuring the solvent-water proton-nuclear magnetic resonance relaxation rate

AU - Aime, Silvio

AU - Dastrú, Walter

AU - Fasano, Mauro

AU - Arnelli, Aldo

AU - Castagnola, Massimo

AU - Giardina, Bruno

AU - Ascenzi, Paolo

PY - 1992

Y1 - 1992

N2 - We report a new method for the quantitative determination of human methemoglobin (metHb) based on the Measurement of the solvent-water proton-nuclear magnetic resonance (NMR) relaxation rate R1 [normalized to 1 mmol/L hemoglobin (Hb) concentration]. MetHb (%) is estimated from the linear dependence of R1 on the metHb concentration, taking into account the simple relationship [MetHb] = [(R1- R1 HbO2)/(R1 metHb - R1 HbO2)] ·100, where RR1 HbO2 and R1 metHb are values for the solvent-water relaxation rate of standard 1.0 mmol/L solutions of the oxygenated derivative of human hemoglobin (HbO2) and of metHb, respectively. The minimum metHb that may be determined from the analysis of the experimental data is 0.5 ± 0.4%.

AB - We report a new method for the quantitative determination of human methemoglobin (metHb) based on the Measurement of the solvent-water proton-nuclear magnetic resonance (NMR) relaxation rate R1 [normalized to 1 mmol/L hemoglobin (Hb) concentration]. MetHb (%) is estimated from the linear dependence of R1 on the metHb concentration, taking into account the simple relationship [MetHb] = [(R1- R1 HbO2)/(R1 metHb - R1 HbO2)] ·100, where RR1 HbO2 and R1 metHb are values for the solvent-water relaxation rate of standard 1.0 mmol/L solutions of the oxygenated derivative of human hemoglobin (HbO2) and of metHb, respectively. The minimum metHb that may be determined from the analysis of the experimental data is 0.5 ± 0.4%.

KW - Hemoglobin

KW - Intermethod comparison

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M3 - Article

C2 - 1458575

AN - SCOPUS:0027049167

SN - 0009-9147

VL - 38

SP - 2401

EP - 2404

JO - Clinical Chemistry

JF - Clinical Chemistry

IS - 12

ER -

Quantitative determination of methemoglobin by measuring the solvent-water proton-nuclear magnetic resonance relaxation rate

Abstract

Keywords

ASJC Scopus subject areas

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