Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients

Fausto Baldanti; Gian Battista Fogazzi; Milena Furione; Lucia Saglimbeni; Francesca Rovida; Marta Gatti; Stefania Paolucci; Antonio Tarantino; Giuseppe Gerna

doi:10.1016/j.diagmicrobio.2006.08.006

Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients

Fausto Baldanti, Gian Battista Fogazzi, Milena Furione, Lucia Saglimbeni, Francesca Rovida, Marta Gatti, Stefania Paolucci, Antonio Tarantino, Giuseppe Gerna

Research output: Contribution to journal › Article › peer-review

Abstract

A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9%) were positive for polyomavirus DNA in blood (median level, 500 copies per milliliter of blood) including all 7 patients with renal function deterioration. Polyomavirus DNA detection in blood for diagnosis of renal function deterioration in KTR showed a sensitivity of 100% and a specificity of 96%, whereas positive and negative predictive values were 50% and 100%, respectively. Diagnostic value of decoy cells detection and polyomavirus DNA quantification in urine samples was negligible.

Original language	English
Pages (from-to)	301-307
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	57
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2006.08.006
Publication status	Published - Mar 2007

Keywords

BK virus
DNA quantification
JC virus
Kidney transplant
PCR
Polyomavirus
Predictive value

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Immunology
Microbiology
Parasitology
Virology
Immunology and Allergy
Infectious Diseases

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10.1016/j.diagmicrobio.2006.08.006

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title = "Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients",

abstract = "A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9%) were positive for polyomavirus DNA in blood (median level, 500 copies per milliliter of blood) including all 7 patients with renal function deterioration. Polyomavirus DNA detection in blood for diagnosis of renal function deterioration in KTR showed a sensitivity of 100% and a specificity of 96%, whereas positive and negative predictive values were 50% and 100%, respectively. Diagnostic value of decoy cells detection and polyomavirus DNA quantification in urine samples was negligible.",

keywords = "BK virus, DNA quantification, JC virus, Kidney transplant, PCR, Polyomavirus, Predictive value",

author = "Fausto Baldanti and Fogazzi, {Gian Battista} and Milena Furione and Lucia Saglimbeni and Francesca Rovida and Marta Gatti and Stefania Paolucci and Antonio Tarantino and Giuseppe Gerna",

year = "2007",

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doi = "10.1016/j.diagmicrobio.2006.08.006",

language = "English",

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pages = "301--307",

journal = "Diagnostic Microbiology and Infectious Disease",

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AU - Baldanti, Fausto

AU - Fogazzi, Gian Battista

AU - Furione, Milena

AU - Saglimbeni, Lucia

AU - Rovida, Francesca

AU - Gatti, Marta

AU - Paolucci, Stefania

AU - Tarantino, Antonio

AU - Gerna, Giuseppe

PY - 2007/3

Y1 - 2007/3

N2 - A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9%) were positive for polyomavirus DNA in blood (median level, 500 copies per milliliter of blood) including all 7 patients with renal function deterioration. Polyomavirus DNA detection in blood for diagnosis of renal function deterioration in KTR showed a sensitivity of 100% and a specificity of 96%, whereas positive and negative predictive values were 50% and 100%, respectively. Diagnostic value of decoy cells detection and polyomavirus DNA quantification in urine samples was negligible.

AB - A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9%) were positive for polyomavirus DNA in blood (median level, 500 copies per milliliter of blood) including all 7 patients with renal function deterioration. Polyomavirus DNA detection in blood for diagnosis of renal function deterioration in KTR showed a sensitivity of 100% and a specificity of 96%, whereas positive and negative predictive values were 50% and 100%, respectively. Diagnostic value of decoy cells detection and polyomavirus DNA quantification in urine samples was negligible.

KW - BK virus

KW - DNA quantification

KW - JC virus

KW - Kidney transplant

KW - PCR

KW - Polyomavirus

KW - Predictive value

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ER -

Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients

Abstract

Keywords

ASJC Scopus subject areas

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