TY - JOUR
T1 - Purification of immunomodulatory factors in human peripheral blood leukocytes
AU - Montecucchi, P. C.
AU - Caldarini, R.
AU - Sansó, M.
AU - Bertolaso, A. M.
AU - Di San Secondo, V. E M Rosso
AU - Piccolo, G.
AU - Sirchia, G.
PY - 1990/7/20
Y1 - 1990/7/20
N2 - Procedure is described for purifying low-molecular-weight factors with antigenaspecific properties from a dialysate of human leukocyte extract. It includes gel chromatography on Sephade G-25 and G-15, ion-exchange chromatography, reversed-phase high-performance liquid chromatography (HPLC) on a C18 hydrophobic column and gel permeation HPLC . The immunosuppressive factor (mol.wt. 800-1000) was purified to near homogeneity. It is probably of peptide nature, although it is pronase resistant. The enhancer factor (mol.wt. 300-600) is eluted from chromatographic columns together with a hypoxanthine-like substance. Nevertheless, the biological activity cannot be attributed to the purine derivative. Identification of this amplifier activity is still lacking.
AB - Procedure is described for purifying low-molecular-weight factors with antigenaspecific properties from a dialysate of human leukocyte extract. It includes gel chromatography on Sephade G-25 and G-15, ion-exchange chromatography, reversed-phase high-performance liquid chromatography (HPLC) on a C18 hydrophobic column and gel permeation HPLC . The immunosuppressive factor (mol.wt. 800-1000) was purified to near homogeneity. It is probably of peptide nature, although it is pronase resistant. The enhancer factor (mol.wt. 300-600) is eluted from chromatographic columns together with a hypoxanthine-like substance. Nevertheless, the biological activity cannot be attributed to the purine derivative. Identification of this amplifier activity is still lacking.
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U2 - 10.1016/S0021-9673(01)89480-2
DO - 10.1016/S0021-9673(01)89480-2
M3 - Article
C2 - 2229224
AN - SCOPUS:0025351963
SN - 0021-9673
VL - 512
SP - 139
EP - 147
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -