TY - JOUR
T1 - Pure uptake blockers of dopamine can reduce prolactin secretion
T2 - Studies with diclofensine
AU - Di Renzo, Gianfranco
AU - Amoroso, Salvatore
AU - Taglialatela, Maurizio
AU - Canzoniero, Lorella M T
AU - Maida, Paola
AU - Lombardi, Gaetano
AU - Annunziato, Lucio
PY - 1988
Y1 - 1988
N2 - The effects of diclofensine, a pure dopamine (DA) uptake inhibitor on 1) 3H-DA uptake in rat arcuate-periventricular nucleus-median eminence synaptosomes, 2) basal and K+-evoked endogenous DA release from tuberoinfundibular dopaminergic (TIDA) neurons and 3) in vivo prolactin (PRL) secretion were studied. Diclofensine, in concentrations of 0.01, 0.1 and 1 μM caused a marked decrease of 3H-DA uptake. In addition, it was unable to stimulate basal endogenous DA release which, on the contrary, was elicited by d-amphetamine in the same concentration (50 μM). On the other hand, diclofensine (50 μM) caused a 3 fold enhancement of K+-evoked DA release. Finally, the compound, when administered in vivo to male rats, significantly reduced basal serum PRL levels. The results of the present study seem to indicate that the pharmacological blockade of DA uptake in TIDA neurons is a condition sufficient to cause a reduction of PRL release.
AB - The effects of diclofensine, a pure dopamine (DA) uptake inhibitor on 1) 3H-DA uptake in rat arcuate-periventricular nucleus-median eminence synaptosomes, 2) basal and K+-evoked endogenous DA release from tuberoinfundibular dopaminergic (TIDA) neurons and 3) in vivo prolactin (PRL) secretion were studied. Diclofensine, in concentrations of 0.01, 0.1 and 1 μM caused a marked decrease of 3H-DA uptake. In addition, it was unable to stimulate basal endogenous DA release which, on the contrary, was elicited by d-amphetamine in the same concentration (50 μM). On the other hand, diclofensine (50 μM) caused a 3 fold enhancement of K+-evoked DA release. Finally, the compound, when administered in vivo to male rats, significantly reduced basal serum PRL levels. The results of the present study seem to indicate that the pharmacological blockade of DA uptake in TIDA neurons is a condition sufficient to cause a reduction of PRL release.
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U2 - 10.1016/0024-3205(88)90131-2
DO - 10.1016/0024-3205(88)90131-2
M3 - Article
C2 - 2968488
AN - SCOPUS:0023914348
SN - 0024-3205
VL - 42
SP - 2161
EP - 2169
JO - Life Sciences
JF - Life Sciences
IS - 21
ER -