Pure uptake blockers of dopamine can reduce prolactin secretion: Studies with diclofensine

Gianfranco Di Renzo, Salvatore Amoroso, Maurizio Taglialatela, Lorella M T Canzoniero, Paola Maida, Gaetano Lombardi, Lucio Annunziato

Research output: Contribution to journalArticlepeer-review


The effects of diclofensine, a pure dopamine (DA) uptake inhibitor on 1) 3H-DA uptake in rat arcuate-periventricular nucleus-median eminence synaptosomes, 2) basal and K+-evoked endogenous DA release from tuberoinfundibular dopaminergic (TIDA) neurons and 3) in vivo prolactin (PRL) secretion were studied. Diclofensine, in concentrations of 0.01, 0.1 and 1 μM caused a marked decrease of 3H-DA uptake. In addition, it was unable to stimulate basal endogenous DA release which, on the contrary, was elicited by d-amphetamine in the same concentration (50 μM). On the other hand, diclofensine (50 μM) caused a 3 fold enhancement of K+-evoked DA release. Finally, the compound, when administered in vivo to male rats, significantly reduced basal serum PRL levels. The results of the present study seem to indicate that the pharmacological blockade of DA uptake in TIDA neurons is a condition sufficient to cause a reduction of PRL release.

Original languageEnglish
Pages (from-to)2161-2169
Number of pages9
JournalLife Sciences
Issue number21
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Pharmacology


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