Protein expression profile of celiac disease patient with aberrant T cell by two-dimensional difference gel electrophoresis

Valli De Re, Maria Paola Simula, Laura Caggiari, Nicoletta Ortz, Michele Spina, Alessandro Da Ponte, Leandro De Appolonia, Riccardo Dolcetti, Vincenzo Canzonieri, Renato Cannizzaro

Research output: Chapter in Book/Report/Conference proceedingConference contribution


One complication of celiac disease (CD) is refractory CD. These patients frequently show aberrant intraepithelial T cell clones and an increasing risk of evolution into enteropathy-associated T cell lymphoma (EATL). There is debate in the literature whether these cases are actually a smoldering lymphoma from the outset. The mechanism inducing T cell proliferation and prognosis remains unknown. Recently, alemtuzumab has been proposed as a promising new approach to treat these patients. Only few single cases have been tested presently, nevertheless, in all of them a clinical improvement has been observed, while intraepithelial lymphocytes (IELs) effectively targeted by alemtuzumab are still a debated issue. Using 2D-DIGE, we found hyperexpressed proteins specifically associated with aberrant T cell in a patient with CD by comparing the protein expression with that of patients with CD and polyclonal T cell or with that of control subjects (patients with polyclonal T cell and no CD). Proteins with a higher expression in duodenal biopsy of the patient with aberrant T cell were identified as IgM, apolipoprotein C-III, and Charcot-Leyden crystal proteins. These preliminary data allow hypothesizing different clinical effects of alemtuzumab in patients with CD, since besides the probable effect of alemtuzumab on T cell, it could effect inflammatory-associated CD52+ IgM+ B cell and eosinophils cells, known to produce IgM and Charcot-Leyden crystal proteins, which we demonstrated to be altered in this patient. Results also emphasize the possible association of apolipoprotein with aberrant T cell proliferation.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Number of pages12
Publication statusPublished - Aug 2007

Publication series

NameAnnals of the New York Academy of Sciences
ISSN (Print)00778923
ISSN (Electronic)17496632


  • 2D-DIGE
  • Celiac disease
  • Enteropathy-associated T cell lymphoma
  • T cell receptor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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