Prostaglandins affect the respiratory burst of human neutrophils

Antonino Sottile; Mario Venza; Isabella Venza; Diana Teti

doi:10.3109/08923979509019753

Prostaglandins affect the respiratory burst of human neutrophils

Antonino Sottile, Mario Venza, Isabella Venza, Diana Teti

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of prostaglandins on superoxide generation by neutrophils were investigated, since these arachidonic acid metabolites are both involved in the early phase of the inflammatory process and during later stages of neutrophil function. Preincubation of these cells for five minutes with concentrations of PGE2 ranging from 10^-7 to 10^-4 M was able to significantly reduce superoxide production in PMA-stimulated neutrophils. Other pro-inflammatory PGs tested, such as PGE_1α, PGF_2α, PGF_2α, inhibited the respiratory burst. the PGE₂-induced inhibition was compared to that exerted by staurosporine, a PKC inhibitor. the effects of the two drugs were not additive, since the combinations of PGE₂ and staurosporine reduced O₂ production to the same extent as staurosporine alone. Possible interferences between PKA-and PKC-mediated transduction signals are discussed.

Original language	English
Pages (from-to)	311-321
Number of pages	11
Journal	Immunopharmacology and Immunotoxicology
Volume	17
Issue number	2
DOIs	https://doi.org/10.3109/08923979509019753
Publication status	Published - 1995

ASJC Scopus subject areas

Immunology
Immunology and Allergy
Pharmacology
Toxicology
Health, Toxicology and Mutagenesis

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abstract = "The effects of prostaglandins on superoxide generation by neutrophils were investigated, since these arachidonic acid metabolites are both involved in the early phase of the inflammatory process and during later stages of neutrophil function. Preincubation of these cells for five minutes with concentrations of PGE2 ranging from 10-7 to 10-4 M was able to significantly reduce superoxide production in PMA-stimulated neutrophils. Other pro-inflammatory PGs tested, such as PGE1α, PGF2α, PGF2α, inhibited the respiratory burst. the PGE2-induced inhibition was compared to that exerted by staurosporine, a PKC inhibitor. the effects of the two drugs were not additive, since the combinations of PGE2 and staurosporine reduced O2 production to the same extent as staurosporine alone. Possible interferences between PKA-and PKC-mediated transduction signals are discussed.",

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Prostaglandins affect the respiratory burst of human neutrophils

Abstract

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