Prognostic role of serum sialyl Lewisx (CD15s) in colorectal cancer

Michela Paganuzzi, Barbara Bobbio, Paola Marroni, Rosa Filiberti, Giovanni Battista Secco, Carlo Enrico Grossi

Research output: Contribution to journalArticlepeer-review


Objective: Sialyl Lewisx (sLex) is one ligand for E selectin (CD62E), a glycoprotein that is expressed on activated endothelial cells. Adhesion mediated by endothelial E selectin and sLex expressed on human tumor cells could be relevant for the development of metastases. The aim of this study was to investigate whether or not a correlation exists between pre-operative levels of ganglioside serum sLex and disease-free interval and survival in colorectal cancer patients. Patients and Methods: Thirty Duke's B and 52 Duke's C patients undergoing resection for colorectal cancer were studied. The median follow-up time was 34.8 months. A pool of 57 sera from normal subjects was used as an Internal Normal Standard (INS). sLex analyses were performed by a thin layer chromatography (TLC) immunostaining technique. Results were expressed as the ratio (R) between bands of INS and bands from each neoplastic serum. Results: The median R value was 0.80 in normal subjects, 0.70 in Duke's B patients and 1.0 in Duke's C patients. No significant differences were detected between sLex concentrations in sera from normal and neoplastic subjects (p = 0.1). Using an arbitrary cutoff of R = 0.9, the mean disease-free interval in the whole series was 47.4 months for R x significantly correlate with a favorable prognosis and with a lower occurrence of metastases. It is conceivable that soluble sLex may compete with membrane-bound sLex in the course of interactions between activated endothelium and tumor cells that have reached the circulation.

Original languageEnglish
Pages (from-to)52-59
Number of pages8
Issue number1
Publication statusPublished - 2003


  • Adhesion molecules
  • CD15s
  • CD62E
  • E selectin
  • Gangliosides
  • sLe
  • Thin layer chromatography

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


Dive into the research topics of 'Prognostic role of serum sialyl Lewisx (CD15s) in colorectal cancer'. Together they form a unique fingerprint.

Cite this