Profiling celiac disease antibody repertoire

Sara D'Angelo, Flavio Mignone, Cecilia Deantonio, Roberto Di Niro, Roberta Bordoni, Roberto Marzari, Gianluca De Bellis, Tarcisio Not, Fortunato Ferrara, Andrew Bradbury, Claudio Santoro, Daniele Sblattero

Research output: Contribution to journalArticlepeer-review


The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research.

Original languageEnglish
Pages (from-to)99-109
Number of pages11
JournalClinical Immunology
Issue number1
Publication statusPublished - Jul 2013


  • Autoantibody
  • Autoantigen
  • Celiac disease
  • Next generation sequencing
  • ORF-display libraries
  • Protein microarray

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy


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