Abstract
This paper reviews the technical aspects of cryoultramicrotomy. The key preparation steps in the technique, namely freezing, sectioning, thawing, and staining are analysed. The application of this method to immunocytochemistry and analytical electron microscopy are discussed with respect to the present possibilities and problems.
| Translated title of the contribution | Principles and application possibilities of cryoultramicrotomy |
|---|---|
| Original language | Italian |
| Pages (from-to) | 249-263 |
| Number of pages | 15 |
| Journal | Annali dell'Istituto Superiore di Sanita |
| Volume | 27 |
| Issue number | 2 |
| Publication status | Published - 1991 |
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health