TY - JOUR
T1 - Preclinical imaging evaluation of mirnas’ delivery and effects in breast cancer mouse models
T2 - A systematic review
AU - Orlandella, Francesca Maria
AU - Auletta, Luigi
AU - Greco, Adelaide
AU - Zannetti, Antonella
AU - Salvatore, Giuliana
N1 - Funding Information:
This research was funded in part by: Ministero dell?Istruzione, dell?Universit? e della Ricerca, Progetti di Ricerca di Rilevante Interesse Nazionale (PRIN)-Bando 2017-grant 2017MHJJ55; Ricerca Corrente, Italian Ministry of Health; MIUR-PON ?Ricerca e Innovazione? 2014?2020 (grant MOLIMONCOBRAIN LAB).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Background: We have conducted a systematic review focusing on the advancements in preclinical molecular imaging to study the delivery and therapeutic efficacy of miRNAs in mouse models of breast cancer. Methods: A systematic review of English articles published in peer-reviewed journals using PubMed, EMBASE, BIOSIS™ and Scopus was performed. Search terms included breast cancer, mouse, mice, microRNA(s) and miRNA(s). Results: From a total of 2073 records, our final data extraction was from 114 manuscripts. The most frequently used murine genetic background was Balb/C (46.7%). The most frequently used model was the IV metastatic model (46.8%), which was obtained via intravenous injection (68.9%) in the tail vein. Bioluminescence was the most used frequently used tool (64%), and was used as a surrogate for tumor growth for efficacy treatment or for the evaluation of tumorigenicity in miRNA-transfected cells (29.9%); for tracking, evaluation of engraftment and for response to therapy in metastatic models (50.6%). Conclusions: This review provides a systematic and focused analysis of all the information available and related to the imaging protocols with which to test miRNA therapy in an in vivo mice model of breast cancer, and has the purpose of providing an important tool to suggest the best preclinical imaging protocol based on available evidence.
AB - Background: We have conducted a systematic review focusing on the advancements in preclinical molecular imaging to study the delivery and therapeutic efficacy of miRNAs in mouse models of breast cancer. Methods: A systematic review of English articles published in peer-reviewed journals using PubMed, EMBASE, BIOSIS™ and Scopus was performed. Search terms included breast cancer, mouse, mice, microRNA(s) and miRNA(s). Results: From a total of 2073 records, our final data extraction was from 114 manuscripts. The most frequently used murine genetic background was Balb/C (46.7%). The most frequently used model was the IV metastatic model (46.8%), which was obtained via intravenous injection (68.9%) in the tail vein. Bioluminescence was the most used frequently used tool (64%), and was used as a surrogate for tumor growth for efficacy treatment or for the evaluation of tumorigenicity in miRNA-transfected cells (29.9%); for tracking, evaluation of engraftment and for response to therapy in metastatic models (50.6%). Conclusions: This review provides a systematic and focused analysis of all the information available and related to the imaging protocols with which to test miRNA therapy in an in vivo mice model of breast cancer, and has the purpose of providing an important tool to suggest the best preclinical imaging protocol based on available evidence.
KW - Breast cancer
KW - Mice models
KW - MiRNAs
KW - Preclinical imaging
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U2 - 10.3390/cancers13236020
DO - 10.3390/cancers13236020
M3 - Review article
AN - SCOPUS:85120052842
SN - 2072-6694
VL - 13
JO - Cancers
JF - Cancers
IS - 23
M1 - 6020
ER -