Postsynaptic effects of methoctramine at the mouse neuromuscular junction

L. Re; V. Cola; G. Fulgenzi; F. Marinelli; C. Concettoni; L. Rossini

doi:10.1016/0306-4522(93)90077-S

Postsynaptic effects of methoctramine at the mouse neuromuscular junction

L. Re, V. Cola, G. Fulgenzi, F. Marinelli, C. Concettoni, L. Rossini

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

Functional studies were performed to evaluate the effects of methoctramine at the neuromuscular junction of the mouse. The presynaptic control of acetylcholine release and the postsynaptic activation of the nicotinic receptor have been analysed by means of the extracellular recording with an EPC7 Patch Clamp amplifier. This electrophysiological method revealed a dose-related inhibitory effect of methoctramine on the studied parameters. The dramatic reduction of the kinetics of the quantal conductance change indicates an action at the postsynaptic level. The effects of methoctramine have been compared with those of the muscarinic agonist oxotremorine. Concentration/response curves for the two drugs were obtained and the apparent ec₅₀ values calculated. The effects of oxotremorine were not antagonized by 1 μM methoctramine. These findings suggest an interaction of some muscarinic agents on the postsynaptic receptor-ion-channel complex at the mouse neuromuscular junction.

Original language	English
Pages (from-to)	451-457
Number of pages	7
Journal	Neuroscience
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/0306-4522(93)90077-S
Publication status	Published - 1993

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0306-4522(93)90077-S

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title = "Postsynaptic effects of methoctramine at the mouse neuromuscular junction",

abstract = "Functional studies were performed to evaluate the effects of methoctramine at the neuromuscular junction of the mouse. The presynaptic control of acetylcholine release and the postsynaptic activation of the nicotinic receptor have been analysed by means of the extracellular recording with an EPC7 Patch Clamp amplifier. This electrophysiological method revealed a dose-related inhibitory effect of methoctramine on the studied parameters. The dramatic reduction of the kinetics of the quantal conductance change indicates an action at the postsynaptic level. The effects of methoctramine have been compared with those of the muscarinic agonist oxotremorine. Concentration/response curves for the two drugs were obtained and the apparent ec50 values calculated. The effects of oxotremorine were not antagonized by 1 μM methoctramine. These findings suggest an interaction of some muscarinic agents on the postsynaptic receptor-ion-channel complex at the mouse neuromuscular junction.",

author = "L. Re and V. Cola and G. Fulgenzi and F. Marinelli and C. Concettoni and L. Rossini",

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T1 - Postsynaptic effects of methoctramine at the mouse neuromuscular junction

AU - Re, L.

AU - Cola, V.

AU - Fulgenzi, G.

AU - Marinelli, F.

AU - Concettoni, C.

AU - Rossini, L.

PY - 1993

Y1 - 1993

N2 - Functional studies were performed to evaluate the effects of methoctramine at the neuromuscular junction of the mouse. The presynaptic control of acetylcholine release and the postsynaptic activation of the nicotinic receptor have been analysed by means of the extracellular recording with an EPC7 Patch Clamp amplifier. This electrophysiological method revealed a dose-related inhibitory effect of methoctramine on the studied parameters. The dramatic reduction of the kinetics of the quantal conductance change indicates an action at the postsynaptic level. The effects of methoctramine have been compared with those of the muscarinic agonist oxotremorine. Concentration/response curves for the two drugs were obtained and the apparent ec50 values calculated. The effects of oxotremorine were not antagonized by 1 μM methoctramine. These findings suggest an interaction of some muscarinic agents on the postsynaptic receptor-ion-channel complex at the mouse neuromuscular junction.

AB - Functional studies were performed to evaluate the effects of methoctramine at the neuromuscular junction of the mouse. The presynaptic control of acetylcholine release and the postsynaptic activation of the nicotinic receptor have been analysed by means of the extracellular recording with an EPC7 Patch Clamp amplifier. This electrophysiological method revealed a dose-related inhibitory effect of methoctramine on the studied parameters. The dramatic reduction of the kinetics of the quantal conductance change indicates an action at the postsynaptic level. The effects of methoctramine have been compared with those of the muscarinic agonist oxotremorine. Concentration/response curves for the two drugs were obtained and the apparent ec50 values calculated. The effects of oxotremorine were not antagonized by 1 μM methoctramine. These findings suggest an interaction of some muscarinic agents on the postsynaptic receptor-ion-channel complex at the mouse neuromuscular junction.

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Postsynaptic effects of methoctramine at the mouse neuromuscular junction

Abstract

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