TY - JOUR
T1 - Pitfalls and promises in FTIR spectromicroscopy analyses to monitor iron-mediated DNA damage in sperm
AU - Pascolo, Lorella
AU - Bedolla, Diana E.
AU - Vaccari, Lisa
AU - Venturin, Irene
AU - Cammisuli, Francesca
AU - Gianoncelli, Alessandra
AU - Mitri, Elisa
AU - Giolo, Elena
AU - Luppi, Stefania
AU - Martinelli, Monica
AU - Zweyer, Marina
AU - Ricci, Giuseppe
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Many drugs, chemicals, and environmental factors can impair sperm functionality by inducing DNA damage, one of the important causes of reduced fertility potential. The use of vibrational spectromicroscopy represents a promising approach for monitoring DNA integrity in sperm, although some limitations exist, depending from the experimental conditions. Here, we report that when using FTIR spectromicroscopy to reveal oxidative stress mediated by Fenton's reaction on hydrated sperm samples, DNA damage interpretation is partially compromised by unexpected cell surface precipitates. The precipitates give a broad band in the 1150-1000 cm-1 infrared region, which partially covers one of the signatures of DNA (phosphate stretching bands), and are detected as iron and oxygen containing material when using XRF spectroscopy. On the other hand, the analyses further support the potential of FTIR spectromicroscopy to reveal cellular oxidative damage events such as lipid peroxidation, protein misfolding and aggregations, as well as DNA strain breaks.
AB - Many drugs, chemicals, and environmental factors can impair sperm functionality by inducing DNA damage, one of the important causes of reduced fertility potential. The use of vibrational spectromicroscopy represents a promising approach for monitoring DNA integrity in sperm, although some limitations exist, depending from the experimental conditions. Here, we report that when using FTIR spectromicroscopy to reveal oxidative stress mediated by Fenton's reaction on hydrated sperm samples, DNA damage interpretation is partially compromised by unexpected cell surface precipitates. The precipitates give a broad band in the 1150-1000 cm-1 infrared region, which partially covers one of the signatures of DNA (phosphate stretching bands), and are detected as iron and oxygen containing material when using XRF spectroscopy. On the other hand, the analyses further support the potential of FTIR spectromicroscopy to reveal cellular oxidative damage events such as lipid peroxidation, protein misfolding and aggregations, as well as DNA strain breaks.
KW - DNA damage
KW - Fenton's reaction
KW - Fourier transform infraRed (FTIR)
KW - Oxidative stress
KW - Phosphate stretching bands
KW - Sperm integrity
KW - Vibrational spectromicroscopy
KW - X-ray fluorescence
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U2 - 10.1016/j.reprotox.2016.02.011
DO - 10.1016/j.reprotox.2016.02.011
M3 - Article
AN - SCOPUS:84959550667
SN - 0890-6238
VL - 61
SP - 39
EP - 46
JO - Reproductigve Toxicoloy
JF - Reproductigve Toxicoloy
ER -