TY - JOUR
T1 - Phylogenetic analysis of HIV type 1 CRF02-AG in multiple genes in Italian and african patients living in Italy
AU - Paolucci, Stefania
AU - Piralla, Antonio
AU - Fiorina, Loretta
AU - Gulminetti, Roberto
AU - Novati, Stefano
AU - Lai, Alessia
AU - Baldanti, Fausto
PY - 2014
Y1 - 2014
N2 - Human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 02-AG is a major recombinant variant in different geographic areas and is predominant in West and Central Africa. Of particular interest is the increased frequency of CRF02-AG in patients living in Italy. In the present study, phylogenetic analyses were performed on gag, pol (integrase), and env (gp120 and gp41) gene sequences from 34 CRF02-AG-infected patients living in Italy. Thirty out of 34 (89.4%) patients were from western Africa, 3/34 (8.8%) were born in Italy, and 1/34 (2.9%) was from Cuba. Phylogenetic analysis revealed the presence of a well-supported clade (aLRT score>0.75) of sequences only in gp120 and gp41 trees. Evolutionary rate estimation showed a faster evolution for the gp120 gene with respect to the gag, integrase, and gp41 genes. This finding was confirmed by the analysis of interpatient variability. Intrapatient variability was greater in gp120 gene sequences; 10/19 (52.6%; p1 as compared with gag, integrase, and gp41 gene sequences with dN/dS ratios
AB - Human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 02-AG is a major recombinant variant in different geographic areas and is predominant in West and Central Africa. Of particular interest is the increased frequency of CRF02-AG in patients living in Italy. In the present study, phylogenetic analyses were performed on gag, pol (integrase), and env (gp120 and gp41) gene sequences from 34 CRF02-AG-infected patients living in Italy. Thirty out of 34 (89.4%) patients were from western Africa, 3/34 (8.8%) were born in Italy, and 1/34 (2.9%) was from Cuba. Phylogenetic analysis revealed the presence of a well-supported clade (aLRT score>0.75) of sequences only in gp120 and gp41 trees. Evolutionary rate estimation showed a faster evolution for the gp120 gene with respect to the gag, integrase, and gp41 genes. This finding was confirmed by the analysis of interpatient variability. Intrapatient variability was greater in gp120 gene sequences; 10/19 (52.6%; p1 as compared with gag, integrase, and gp41 gene sequences with dN/dS ratios
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U2 - 10.1089/aid.2014.0002
DO - 10.1089/aid.2014.0002
M3 - Article
C2 - 24892582
AN - SCOPUS:84905455973
SN - 0889-2229
VL - 30
SP - 812
EP - 818
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 8
ER -